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  • KD/KO Validated

C9orf72 Polyclonal antibody

C9orf72 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 22637-1-AP

Synonyms

C9orf72, C9RANT, RP11 27J8.2



Tested Applications

Positive WB detected inrat brain tissue, transfected cells, SH-SY5Y cells, HeLa cells, Sp2/0 cells, mouse testis tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22637-1-AP is C9orf72 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity Caenorhabditis elegans, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf72 fusion protein Ag18326
Full Name chromosome 9 open reading frame 72
Calculated molecular weight 481 aa, 54 kDa
Observed molecular weight 50-54 kDa, 25-30 kDa, 43 kDa, 68-72 kDa
GenBank accession numberBC020851
Gene symbol C9orf72
Gene ID (NCBI) 203228
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. It has been reported that C9orf72 forms a complex with Cofilin and other actin binding proteins and 22637-1-AP antibody detects the complex bands around 43 and 68-72 kDa in SDS-PAGE.(PMID: 27723745)

Protocols

Product Specific Protocols
WB protocol for C9orf72 antibody 22637-1-APDownload protocol
IHC protocol for C9orf72 antibody 22637-1-APDownload protocol
IF protocol for C9orf72 antibody 22637-1-APDownload protocol
IP protocol for C9orf72 antibody 22637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Neuropathol Commun

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.

Authors - Peter M Sullivan
  • KO Validated
humanWB

Acta Neuropathol

Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.

Authors - Shahram Saberi
mouseWB

Cells

C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner.

Authors - Stina Leskelä
  • KD Validated
Caenorhabditis elegansWB

PLoS Genet

C9orf72/ALFA-1 controls TFEB/HLH-30-dependent metabolism through dynamic regulation of Rag GTPases.

Authors - Yon Ju Ji
  • KD Validated
humanWB

Acta Neuropathol Commun

C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.

Authors - John L Goodier
mouseWB,IF

J Anat

Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Authors - Ross Ferguson