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C9orf72 Polyclonal antibody
C9orf72 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 22637-1-AP
|Positive WB detected in||rat brain tissue, transfected cells, SH-SY5Y cells, HeLa cells, Sp2/0 cells, mouse testis tissue|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human gliomas tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22637-1-AP is C9orf72 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Caenorhabditis elegans, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C9orf72 fusion protein Ag18326|
|Full Name||chromosome 9 open reading frame 72|
|Calculated molecular weight||481 aa, 54 kDa|
|Observed molecular weight||50-54 kDa, 25-30 kDa, 43 kDa, 68-72 kDa|
|GenBank accession number||BC020851|
|Gene ID (NCBI)||203228|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. It has been reported that C9orf72 forms a complex with Cofilin and other actin binding proteins and 22637-1-AP antibody detects the complex bands around 43 and 68-72 kDa in SDS-PAGE.(PMID: 27723745)
|Product Specific Protocols|
|WB protocol for C9orf72 antibody 22637-1-AP||Download protocol|
|IHC protocol for C9orf72 antibody 22637-1-AP||Download protocol|
|IF protocol for C9orf72 antibody 22637-1-AP||Download protocol|
|IP protocol for C9orf72 antibody 22637-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Acta Neuropathol Commun
The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.
Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.
C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner.
C9orf72/ALFA-1 controls TFEB/HLH-30-dependent metabolism through dynamic regulation of Rag GTPases.
Acta Neuropathol Commun
C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.
Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.