Tested Applications
| Positive WB detected in | human brain tissue, C6 cells, Neuro-2a cells |
| Positive IP detected in | C6 cells |
| Positive IHC detected in | human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66140-1-Ig targets C9orf72 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21080 Product name: Recombinant human C9orf72 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-169 aa of BC020851 Sequence: MSTLCPPPSPAVAKTEIALSGKSPLLAATFAYWDNILGPRVRHIWAPKTEQVLLSDGEITFLANHTLNGEILRNAESGAIDVKFFVLSEKGVIIVSLIFDGNWNGDRSTYGLSIILPQTELSFYLPLHRVCVDRLTHIIRKGRIWMHKERQENVQKIILEGTERMEDQG Predict reactive species |
| Full Name | chromosome 9 open reading frame 72 |
| Calculated Molecular Weight | 481 aa, 54 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC020851 |
| Gene Symbol | C9orf72 |
| Gene ID (NCBI) | 203228 |
| RRID | AB_2784547 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96LT7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
| IHC protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
| IP protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
| WB protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C9ORF72 antibody (Cat# 66140-1-Ig) has 6 total citations published in Nature Neuroscience, Acta Neuropathologica, Acta Neuropathologica Communications, Scientific Reports, Animal Models and Experimental Medicine, and Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration. Research fields include amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration, C9orf72 hexanucleotide repeat expansion, actin dynamics in motor neurons, and neurodegeneration. Applications span WB, IHC, and IF in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis. | ||
Acta Neuropathol Commun Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers. | ||
Sci Rep Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo. | ||
Animal Model Exp Med Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats. | ||
Amyotroph Lateral Scler Frontotemporal Degener Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies. |
