|Positive WB detected in||HEK-293 cells, human stomach tissue, mouse colon tissue, mouse kidney tissue, mouse ovary tissue, rat kidney tissue, U2OS cells|
|Positive IP detected in||mouse kidney tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
16961-1-AP targets CA2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CA2 fusion protein Ag10680|
|Full Name||carbonic anhydrase II|
|Calculated molecular weight||260 aa, 29 kDa|
|Observed molecular weight||29 kDa|
|GenBank accession number||BC011949|
|Gene ID (NCBI)||760|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CA2(carbonic anhydrase II ) is also named as Car2, CA-II, CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in the development of brain(PMID:16825953). Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3).
Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation.
Front Mol Neurosci
Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.
SWATH-based proteomics identified carbonic anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma.
Mol Cell Proteomics
Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Isabel (Verified Customer) (07-03-2020)
Antibody was used to detect carbonic anhydrase II in protein samples taken from primary mouse osteoclasts. The blot produced was really clean with no other bands.