Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human stomach tissue, mouse colon tissue, mouse kidney tissue, mouse ovary tissue, rat kidney tissue, U2OS cells|
|Positive IP detected in||mouse kidney tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
16961-1-AP targets CA2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CA2 fusion protein Ag10680|
|Full Name||carbonic anhydrase II|
|Calculated molecular weight||260 aa, 29 kDa|
|Observed molecular weight||29 kDa|
|GenBank accession number||BC011949|
|Gene ID (NCBI)||760|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CA2(carbonic anhydrase II ) is also named as Car2, CA-II, CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in the development of brain(PMID:16825953). Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3).
Mol Cell Proteomics
Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.
SWATH-based proteomics identified carbonic anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma.
J Inflamm Res
M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs
Front Mol Neurosci
Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.
Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network.
Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Adrianna (Verified Customer) (10-31-2022)
Very good antibody, 1:1000 dilution incubated ON at 4 deg C gave a strong signal.
Isabel (Verified Customer) (07-03-2020)
Antibody was used to detect carbonic anhydrase II in protein samples taken from primary mouse osteoclasts. The blot produced was really clean with no other bands.