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CaMKII alpha-Specific Polyclonal antibody
CaMKII alpha-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 20666-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue, SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20666-1-AP targets CaMKII alpha-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||calcium/calmodulin-dependent protein kinase II alpha|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||NM_015981|
|Gene ID (NCBI)||815|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CaMKII alpha, also named as CAMKA and KIAA0968, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody is specific to CAMK2A.
|Product Specific Protocols|
|WB protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|IHC protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|IF protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|IP protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.
ACS Chem Neurosci
Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.
Sci Total Environ
Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.
Int J Biol Sci
Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression.
Proteomic Profile of Carbonylated Proteins Screen the Regulation of Calmodulin-Dependent Protein Kinases-AMPK-Beclin1 in Aerobic Exercise-Induced Autophagy in Middle-Aged Rat Hippocampus.
J Neuropathol Exp Neurol
Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (05-31-2021)
Human primary cortical cells went trough ICC and the CaMKII antibody at the 1/50 dilution was used overnight at 4°C.
Jonathan (Verified Customer) (11-11-2019)
Works well for WB (1:1000)