CaMKII alpha-Specific Polyclonal antibody

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  WB analysis of HEK-293 using 20666-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20666-1-AP (CaMKII alpha-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20666-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20666-1-AP (CaMKII alpha-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20666-1-AP

  IP result of anti-CaMKII alpha-Specific (IP:20666-1-AP, 4ug; Detection:20666-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

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  IHC staining of human brain using 20666-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20666-1-AP (CaMKII alpha-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20666-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20666-1-AP (CaMKII alpha-Specific antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 20666-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20666-1-AP (CaMKII alpha-Specific antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 20666-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20666-1-AP (CaMKII alpha-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CaMKII alpha-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 20666-1-AP

Publications(12)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMK2, CAMKA, CaMKII alpha

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

20666-1-AP targets CaMKII alpha-Specific in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: calcium/calmodulin-dependent protein kinase II alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_015981
• Gene Symbol: CaMKII alpha
• Gene ID (NCBI): 815
• RRID: AB_2878722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UQM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMKII alpha, also named CAMKA and KIAA0968, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody is specific to CAMK2A.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong ; KD Validated
	WB	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu
human	WB	Int J Biol Sci; Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression.; Authors - Qing Liu
	WB	Gerontology; Proteomic Profile of Carbonylated Proteins Screen the Regulation of Calmodulin-Dependent Protein Kinases-AMPK-Beclin1 in Aerobic Exercise-Induced Autophagy in Middle-Aged Rat Hippocampus.; Authors - Wenfeng Liu
	WB	J Neuropathol Exp Neurol; Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.; Authors - Wenfeng Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the CaMKII antibody at the 1/50 dilution was used overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human primary cortical cells

CaMKII alpha-Specific Antibody Immunohistochemistry validation (1/50 dilution) in Human primary cortical cells (Cat no:20666-1-AP)

FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB (1:1000)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000