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- KD/KO Validated
CaMKII alpha-Specific Polyclonal antibody
CaMKII alpha-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 20666-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue, SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 10 publications below|
|CoIP||See 1 publications below|
20666-1-AP targets CaMKII alpha-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||calcium/calmodulin-dependent protein kinase II alpha|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||NM_015981|
|Gene ID (NCBI)||815|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CaMKII alpha, also named as CAMKA and KIAA0968, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody is specific to CAMK2A.
|Product Specific Protocols|
|WB protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|IHC protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|IF protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|IP protocol for CaMKII alpha-Specific antibody 20666-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Sci Total Environ
Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.
Int J Biol Sci
Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression.
Proteomic Profile of Carbonylated Proteins Screen the Regulation of Calmodulin-Dependent Protein Kinases-AMPK-Beclin1 in Aerobic Exercise-Induced Autophagy in Middle-Aged Rat Hippocampus.
J Neuropathol Exp Neurol
Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.
Regular aerobic exercise-ameliorated troponin I carbonylation to mitigate aged rat soleus muscle functional recession.
ACS Chem Neurosci
Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (05-31-2021)
Human primary cortical cells went trough ICC and the CaMKII antibody at the 1/50 dilution was used overnight at 4°C.
Jonathan (Verified Customer) (11-11-2019)
Works well for WB (1:1000)