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  • KD/KO Validated

CaMKII alpha-Specific Polyclonal antibody

CaMKII alpha-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 20666-1-AP


CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMKA, CaMKII alpha, CaMKII alpha-Specific, KIAA0968

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, SH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20666-1-AP targets CaMKII alpha-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium/calmodulin-dependent protein kinase II alpha
Calculated molecular weight 55 kDa
Observed molecular weight 50 kDa
GenBank accession numberNM_015981
Gene symbol CAMK2A
Gene ID (NCBI) 815
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CaMKII alpha, also named as CAMKA and KIAA0968, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody is specific to CAMK2A.


Product Specific Protocols
WB protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IHC protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IF protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IP protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biomed Res Int

Proteomic Profile of Carbonylated Proteins Screen Regulation of Apoptosis via CaMK Signaling in Response to Regular Aerobic Exercise.

Authors - Wenfeng Liu

Dis Model Mech

Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities.

Authors - Michelle Stewart

ACS Chem Neurosci

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Authors - Lei Wu

Cell Death Dis

Vezatin regulates seizures by controlling AMPAR-mediated synaptic activity.

Authors - You Wang

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
  • KD Validated

J Neuropathol Exp Neurol

Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.

Authors - Wenfeng Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the CaMKII antibody at the 1/50 dilution was used overnight at 4°C.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: Human primary cortical cells
CaMKII alpha-Specific Antibody Immunohistochemistry validation (1/50 dilution) in Human primary cortical cells (Cat no:20666-1-AP)

Jonathan (Verified Customer) (11-11-2019)

Works well for WB (1:1000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000