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- KD/KO Validated
CaMKII Beta Polyclonal antibody
CaMKII Beta Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 11533-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, SH-SY5Y cells, human brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11533-1-AP targets CaMKII Beta in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebra finches|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CaMKII Beta fusion protein Ag2119|
|Full Name||calcium/calmodulin-dependent protein kinase II beta|
|Calculated molecular weight||503 aa, 56 kDa|
|Observed molecular weight||50-70 kDa|
|GenBank accession number||BC019070|
|Gene ID (NCBI)||816|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named as CAM2, CAMK2 and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target which can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with the molecular weight between 50 kDa and 73 kDa. This antibody may have cross reaction with CAMK2A/D/G due to the high homology.
Sci Total Environ
Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.
Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.
Front Cell Neurosci
Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.
Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice.
Behav Brain Res
Social context-dependent singing alters molecular markers of synaptic plasticity signaling in finch basal ganglia Area X.
Aging (Albany NY)
Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.