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  • KD/KO Validated

CaMKII Beta Polyclonal antibody

CaMKII Beta Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11533-1-AP

Synonyms

CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2, CAMK2B, CAMKB, CaMKII beta



Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11533-1-AP targets CaMKII Beta in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebra finches
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CaMKII Beta fusion protein Ag2119
Full Name calcium/calmodulin-dependent protein kinase II beta
Calculated molecular weight 503 aa, 56 kDa
Observed molecular weight 50-70 kDa
GenBank accession numberBC019070
Gene symbol CAMK2B
Gene ID (NCBI) 816
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named as CAM2, CAMK2 and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target which can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with the molecular weight between 50 kDa and 73 kDa. This antibody may have cross reaction with CAMK2A/D/G due to the high homology.

Protocols

Product Specific Protocols
WB protocol for CaMKII Beta antibody 11533-1-APDownload protocol
IHC protocol for CaMKII Beta antibody 11533-1-APDownload protocol
IP protocol for CaMKII Beta antibody 11533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Total Environ

Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.

Authors - Huanliang Liu
humanWB,IHC

Front Oncol

Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.

Authors - Qingan Jia
  • KD Validated
humanIP

Front Cell Neurosci

Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.

Authors - Shuchao Wang
mouseWB

PLoS One

Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice.

Authors - Kathy Busse
zebra finches WB

Behav Brain Res

Social context-dependent singing alters molecular markers of synaptic plasticity signaling in finch basal ganglia Area X.

Authors - Lisa Y So
ratWB

Aging (Albany NY)

Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.

Authors - Ting-Ting Chen