- Featured Product
- KD/KO Validated
CaMKII Beta Polyclonal antibody
CaMKII Beta Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 11533-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, SH-SY5Y cells, human brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11533-1-AP targets CaMKII Beta in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig, zebra finches|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CaMKII Beta fusion protein Ag2119|
|Full Name||calcium/calmodulin-dependent protein kinase II beta|
|Calculated molecular weight||503 aa, 56 kDa|
|Observed molecular weight||50-70 kDa|
|GenBank accession number||BC019070|
|Gene ID (NCBI)||816|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named as CAM2, CAMK2 and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target which can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with the molecular weight between 50 kDa and 73 kDa. This antibody may have cross reaction with CAMK2A/D/G due to the high homology.
Aging (Albany NY)
Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.
Sci Total Environ
Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.
Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.
Front Cell Neurosci
Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.
Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice.
Ketamine Induces Lasting Antidepressant Effects by Modulating the NMDAR/CaMKII-Mediated Synaptic Plasticity of the Hippocampal Dentate Gyrus in Depressive Stroke Model.