Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11533-1-AP targets CAMK2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebra finches |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2119 Product name: Recombinant human CAMK2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC019070 Sequence: MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFS Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase II beta |
| Calculated Molecular Weight | 503 aa, 56 kDa |
| Observed Molecular Weight | 50-70 kDa |
| GenBank Accession Number | BC019070 |
| Gene Symbol | CaMKII beta |
| Gene ID (NCBI) | 816 |
| RRID | AB_2275067 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13554 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CAMK2B antibody 11533-1-AP | Download protocol |
| IP protocol for CAMK2B antibody 11533-1-AP | Download protocol |
| WB protocol for CAMK2B antibody 11533-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CAMK2B Polyclonal antibody (Cat# 11533-1-AP) has 18 citations spanning journals including Theranostics, Advanced Science, Journal of Ethnopharmacology, Food & Function, Aging, European Journal of Pharmacology, Science of the Total Environment, Toxics, Oncology Research, Frontiers in Cell and Developmental Biology, Frontiers in Oncology, Neural Plasticity, Neuroscience, Biomedical Research International, Behavioural Brain Research, Journal of Anesthesia, PLoS ONE, and Liver International. Research fields cover spinal cord injury, ferroptosis, depression, glioma, renal cell carcinoma, vascular dementia, synaptic plasticity, aortic calcification, neurotoxicity, liver fibrosis, and skeletal muscle biology.
| Species | Application | Title |
|---|---|---|
Theranostics Biomimetic piezoelectric hydrogel system for energy metabolism reprogramming in spinal cord injury repair | ||
Adv Sci (Weinh) Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress-Induced Depression in Male Mice. | ||
J Ethnopharmacol Jiawei Buzhong Yiqi decoction ameliorates polycystic ovary syndrome via oocyte-granulosa cell communication | ||
Food Funct Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs | ||
Aging (Albany NY) Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway. | ||
Eur J Pharmacol Piezo1 activation mediates stiffness-induced aortic medial calcification: Pharmacological evidence from agonist and antagonist studies. |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The reviewer used it for Western Blot on liver tissue at a 1:1000 dilution and described it as "best for Western Blot," indicating strong performance and reliability in that application. Overall, the feedback is positive, highlighting the antibody's effectiveness for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-07-2025) | BEST FOR WESTERN BLOT
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