Calnexin Polyclonal antibody

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  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10427-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10427-2-AP

  WB result of Calnexin antibody (10427-2-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells.

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  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10427-2-AP (Calnexin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10427-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 10427-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was stained with CL594-Phalloidin (red).

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  IF Staining of SKOV-3 using 10427-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using Calnexin antibody (10427-2-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 10427-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 10427-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10427-2-AP (Calnexin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10427-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10427-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calnexin (10427-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Calnexin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10427-2-AP

Publications(495)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CANX, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90, CNX

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, RAW 264.7 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, SKOV-3 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 423 publications below
• IHC: See 4 publications below
• IF: See 71 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

10427-2-AP targets Calnexin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, hamster, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calnexin fusion protein Ag0697
• Full Name: calnexin
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC003552
• Gene Symbol: Calnexin
• Gene ID (NCBI): 821
• RRID: AB_2069033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27824
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nature; A virally encoded high-resolution screen of cytomegalovirus dependencies; Authors - Yaara Finkel
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Cell Res; Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies; Authors - Liang Guo
human	WB	Mol Cancer; Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity; Authors - Can Can
human	WB	Cell Discov; A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma; Authors - Xifei Jiang
		Immunity; The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity; Authors - Tian Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guo-rong (Verified Customer) (02-06-2023)

Clear bands can be detected in total cell lysates rather than in extracellular vesicles, as a good EV exclusive marker

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

Calnexin Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:10427-2-AP)

FH

Saba (Verified Customer) (03-14-2022)

The band of the antibody was so sharp and specific. The dilution I used was 1:4000 and it worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000

FH

Meliti (Verified Customer) (09-25-2019)

1:1000 dilution in 5%milk PBST O/N incubation for all antibodies depicted which all come from Proteintech 1:5000 anti rabbit HRP in 5%milk PBST for 1h

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human HEK293 WCEs and exosomes released from these cells and purified by size exclusion chromatography

Calnexin Antibody Western Blot, validation (1:1000 dilution) in Human HEK293 WCEs and exosomes released from these cells and purified by size exclusion chromatography (Cat no:10427-2-AP)