Calnexin Polyclonal antibody
Calnexin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
153
Cat no : 10427-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, SKOV-3 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 131 publications below |
| IHC | See 1 publications below |
| IF | See 27 publications below |
| ELISA | See 1 publications below |
Product Information
10427-2-AP targets Calnexin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat, Hamster, monkey, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Calnexin fusion protein Ag0697 |
| Full Name | calnexin |
| Calculated molecular weight | 90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC003552 |
| Gene symbol | CANX |
| Gene ID (NCBI) | 821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Calnexin antibody 10427-2-AP | Download protocol |
| IHC protocol for Calnexin antibody 10427-2-AP | Download protocol |
| IF protocol for Calnexin antibody 10427-2-AP | Download protocol |
| FC protocol for Calnexin antibody 10427-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioconjug Chem Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales. | ||
Int J Mol Med Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway. | ||
Int J Mol Sci Exosome-Mediated Delivery of Inducible miR-423-5p Enhances Resistance of MRC-5 Cells to Rabies Virus Infection. | ||
Stem Cell Res Ther Exosomes secreted by mesenchymal stromal/stem cell-derived adipocytes promote breast cancer cell growth via activation of Hippo signaling pathway. | ||
J Cell Mol Med Two novel ANK1 loss-of-function mutations in Chinese families with hereditary spherocytosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Saba (Verified Customer) (03-14-2022) | The band of the antibody was so sharp and specific. The dilution I used was 1:4000 and it worked perfectly.
|
FH Meliti (Verified Customer) (09-25-2019) | 1:1000 dilution in 5%milk PBST O/N incubation for all antibodies depicted which all come from Proteintech 1:5000 anti rabbit HRP in 5%milk PBST for 1h
 |
