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Calnexin Polyclonal antibody
Calnexin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
374
Cat no : 10427-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, RAW 264.7 cells |
Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, SKOV-3 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 324 publications below |
IHC | See 3 publications below |
IF | See 56 publications below |
ELISA | See 1 publications below |
CoIP | See 1 publications below |
Product Information
10427-2-AP targets Calnexin in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, hamster, goat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Calnexin fusion protein Ag0697 |
Full Name | calnexin |
Calculated Molecular Weight | 90 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC003552 |
Gene Symbol | Calnexin |
Gene ID (NCBI) | 821 |
RRID | AB_2069033 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.
Protocols
Product Specific Protocols | |
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WB protocol for Calnexin antibody 10427-2-AP | Download protocol |
IHC protocol for Calnexin antibody 10427-2-AP | Download protocol |
IF protocol for Calnexin antibody 10427-2-AP | Download protocol |
FC protocol for Calnexin antibody 10427-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies | ||
Cell Discov A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma | ||
Immunity The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity | ||
Nat Microbiol Modulation of membrane phosphoinositide dynamics by the phosphatidylinositide 4-kinase activity of the Legionella LepB effector. | ||
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Guo-rong (Verified Customer) (02-06-2023) | Clear bands can be detected in total cell lysates rather than in extracellular vesicles, as a good EV exclusive marker
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FH Saba (Verified Customer) (03-14-2022) | The band of the antibody was so sharp and specific. The dilution I used was 1:4000 and it worked perfectly.
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FH Meliti (Verified Customer) (09-25-2019) | 1:1000 dilution in 5%milk PBST O/N incubation for all antibodies depicted which all come from Proteintech 1:5000 anti rabbit HRP in 5%milk PBST for 1h
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