Calpain 9 Polyclonal antibody

Calpain 9 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17556-1-AP

Synonyms

Calpain 9, CAPN9, GC36, nCL 4, NCL4, New calpain 4, Protein CG36



Tested Applications

Positive WB detected inhuman stomach tissue, HL-60 cells, mouse small intestine tissue
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human stomach tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17556-1-AP targets Calpain 9 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 9 fusion protein Ag11718
Full Name calpain 9
Calculated molecular weight 627 aa, 72 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC027993
Gene symbol CAPN9
Gene ID (NCBI) 10753
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).

Protocols

Product Specific Protocols
WB protocol for Calpain 9 antibody 17556-1-APDownload protocol
IHC protocol for Calpain 9 antibody 17556-1-APDownload protocol
IF protocol for Calpain 9 antibody 17556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Sci Rep

Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer.

Authors - Peike Peng