|Positive WB detected in||HeLa cells, Apoptosised|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human endometrial cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14311-1-AP targets Caspase 10/p23/17/p12 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caspase 10/p23/17/p12 fusion protein Ag5511|
|Full Name||caspase 10, apoptosis-related cysteine peptidase|
|Calculated molecular weight||59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC042844|
|Gene ID (NCBI)||843|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caspase 10 (CASP10) is also called MCH4, is a member of the peptidase C14A family, and contains two death
effector (DED) domains.
What is the molecular weight of CASP10?
The molecular weight of CASP10 observed on Western blots may vary depending on which of its isoform,
truncation, or cleavage forms are present. Full-length CASP10 has a propeptide with 219 amino acid and
6 isoforms produced by alternative splicing.
What is the tissue specificity of CASP10?
CASP10 is detectable in most tissues. However, there is lower expression observed in brain, kidney, colon,
prostate, and testis tissue.
What is the function of CASP10?
CASP10 plays a role in the cascade of caspases responsible for executing apoptosis. It is recruited to both the
Fas and TNFR-1 receptors in a FADD-dependent manner. CASP10 is responsible for the cleavage and activation of
caspases 3, 4, 6, 7, 8, and 9. CASP10 can be cleaved by granzyme B and autocatalytic activity to generate two
active subunits from its inactive proenzyme form.
What is the role of CASP10 in disease?
Defects in CASP10 lead to autoimmune lymphoproliferative syndrome type 2A (ALPS2A), which is characterized
by abnormal lymphocyte and dendritic cell homeostasis, as well as immune regulatory defects (PMID:16446975).
Defects in CASP10 are also a cause of familial non-Hodgkin lymphoma (NHL) and often marked by enlarged lymph
nodes, weight loss and fever (PMID:12010812), and gastric cancers (GASC) (PMID:11973654).
|Product Specific Protocols|
|WB protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP||Download protocol|
|IHC protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP||Download protocol|
|IF protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP||Download protocol|
|IP protocol for Caspase 10/p23/17/p12 antibody 14311-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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