Caspase 10/p23/17/p12 Polyclonal antibody

Caspase 10/p23/17/p12 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14311-1-AP

Synonyms

ALPS2, Apoptotic protease Mch 4, CASP 10, CASP10, Caspase 10, Caspase 10/p23/17/p12, Caspase10, FLICE2, ICE like apoptotic protease 4, MCH4



Tested Applications

Positive WB detected inHeLa cells, Apoptosised
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14311-1-AP targets Caspase 10/p23/17/p12 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 10/p23/17/p12 fusion protein Ag5511
Full Name caspase 10, apoptosis-related cysteine peptidase
Calculated molecular weight 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC042844
Gene symbol CASP10
Gene ID (NCBI) 843
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 10 (CASP10) is also called MCH4, is a member of the peptidase C14A family, and contains two death 

effector (DED) domains.


What is the molecular weight of CASP10?

The molecular weight of CASP10 observed on Western blots may vary depending on which of its isoform, 

truncation, or cleavage forms are present. Full-length CASP10 has a propeptide with 219 amino acid and 

6 isoforms produced by alternative splicing.


What is the tissue specificity of CASP10?

CASP10 is detectable in most tissues. However, there is lower expression observed in brain, kidney, colon, 

prostate, and testis tissue.


What is the function of CASP10?

CASP10 plays a role in the cascade of caspases responsible for executing apoptosis. It is recruited to both the 

Fas and TNFR-1 receptors in a FADD-dependent manner. CASP10 is responsible for the cleavage and activation of 

caspases 3, 4, 6, 7, 8, and 9. CASP10 can be cleaved by granzyme B and autocatalytic activity to generate two 

active subunits from its inactive proenzyme form.


What is the role of CASP10 in disease?

Defects in CASP10 lead to autoimmune lymphoproliferative syndrome type 2A (ALPS2A), which is characterized 

by abnormal lymphocyte and dendritic cell homeostasis, as well as immune regulatory defects (PMID:16446975). 

Defects in CASP10 are also a cause of familial non-Hodgkin lymphoma (NHL) and often marked by enlarged lymph 

nodes, weight loss and fever (PMID:12010812), and gastric cancers (GASC) (PMID:11973654).


Protocols

Product Specific Protocols
WB protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
IHC protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
IF protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
IP protocol for Caspase 10/p23/17/p12 antibody 14311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols