Caspase 12 Polyclonal antibody

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• Caspase 12 Polyclonal antibody (55238-1-AP)

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Caspase 12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55238-1-AP Copied!

Publications (123)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CASP 12, CASP12, CASP12P1, Caspase 12, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12

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  WB analysis of HEK-293 using 55238-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of multi-cells using 55238-1-AP

  WB result of 55238-1-AP (CASP12 antibody) with various lysates.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 55238-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 55238-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 55238-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 55238-1-AP

  IP result of anti-Caspase 12 (IP:55238-1-AP, 4ug; Detection:55238-1-AP 1:1000) with HEK-293 cells lysate 2000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 55238-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55238-1-AP (Caspase 12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, multi-cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate hyperplasia tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55238-1-AP targets Caspase 12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 12 (gene/pseudogene)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 36-42 kDa, 50 kDa
• GenBank Accession Number: NR_000035
• Gene Symbol: Caspase 12
• Gene ID (NCBI): 120329
• RRID: AB_10892670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.

Publications

What published studies show

The Caspase 12 Polyclonal antibody (Cat# 55238-1-AP) has 123 citations. These appear across journals including Bioactive Materials, Autophagy, Theranostics, Advanced Science, Cell Death & Disease, EBioMedicine, Journal of Medicinal Chemistry, and Toxics. Research fields span endoplasmic reticulum (ER) stress, apoptosis, autophagy, and the unfolded protein response, applied to cancer (breast, lung, pancreatic, nasopharyngeal), neurotoxicity, cardioprotection, renal injury, and environmental toxicology. Most studies use Western blot in human samples.

Automatically generated from the 123 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Bioact Mater; Novel neutrophil extracellular trap-related mechanisms in diabetic wounds inspire a promising treatment strategy with hypoxia-challenged small extracellular vesicles; Authors - Ziqiang Chu
human	WB	Autophagy; SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.; Authors - Xiaolin Zhang
human	WB,IF	Theranostics; Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.; Authors - Zhiwei Liao
		Adv Sci (Weinh); Intrinsically Mitochondria-Targeting Nanozyme via Coordination-Assembly of Natural Quercetin for Cascade Antioxidant Therapy of Cerebral Ischemia-Reperfusion Injury.; Authors - Wenxuan Zheng
human	WB	Asian J Pharm Sci; Enhanced photodynamic therapy/photothermo therapy for nasopharyngeal carcinoma via a tumour microenvironment-responsive self-oxygenated drug delivery system.; Authors - Nan Wu
human	WB	Cell Death Dis; Betulinic acid chemosensitizes breast cancer by triggering ER stress-mediated apoptosis by directly targeting GRP78.; Authors - Youli Cai