Validation Data Gallery
|Positive WB detected in||HEK-293 cells, multi-cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human prostate hyperplasia tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55238-1-AP targets Caspase 12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||hamster, human|
|Host / Isotype||Rabbit / IgG|
|Full Name||caspase 12 (gene/pseudogene)|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||36-42 kDa, 50 kDa|
|GenBank accession number||NR_000035|
|Gene ID (NCBI)||120329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.
|Product Specific Protocols|
|WB protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IHC protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IF protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IP protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Knockdown MTMR14 promotes cell apoptosis and inhibits migration in liver cancer cells.
J Cell Physiol
Sensory plasticity of carotid body is correlated with oxidative stress in paraventricular nucleus during chronic intermittent hypoxia.
Oxid Med Cell Longev
Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca2+ Imbalance-Mediated Endoplasmic Reticulum Stress.
Int J Mol Sci
Bone marrow mesenchymal stem cells could reduce the toxic effects of hexavalent chromium on the liver by decreasing endoplasmic reticulum stress-mediated apoptosis via SIRT1/HIF-1α signaling pathway in rats.
HBOA ameliorates CCl4-incuded liver fibrosis through inhibiting TGF-β1/Smads, NF-κB and ERK signaling pathways.