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- KD/KO Validated
Caspase 12 Polyclonal antibody
Caspase 12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (2)
WB, IP, IHC, IF, ELISA
Cat no : 55238-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, multi-cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human prostate hyperplasia tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 77 publications below|
|IHC||See 4 publications below|
|IF||See 4 publications below|
55238-1-AP targets Caspase 12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||caspase 12 (gene/pseudogene)|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||36-42 kDa, 50 kDa|
|GenBank accession number||NR_000035|
|Gene ID (NCBI)||120329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.
|Product Specific Protocols|
|WB protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IHC protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IF protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IP protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|Click here to view our Standard Protocols|
SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.
J Hazard Mater
Perinatal low-dose PBDE-47 exposure hampered thyroglobulin turnover and induced thyroid cell apoptosis by triggering ER stress and lysosomal destabilization contributing to thyroid toxicity in adult female rats.
Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.
J Med Chem
Anticancer Effects of Honokiol via Mitochondrial Dysfunction Are Strongly Enhanced by the Mitochondria-Targeting Carrier Berberine.
Cell Death Dis
Betulinic acid chemosensitizes breast cancer by triggering ER stress-mediated apoptosis by directly targeting GRP78.
J Mol Cell Cardiol
TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.