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Caspase 12 Polyclonal antibody

Caspase 12 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 55238-1-AP


CASP 12, CASP12, CASP12P1, Caspase 12, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12

Tested Applications

Positive WB detected inHEK-293 cells, multi-cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate hyperplasia tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55238-1-AP targets Caspase 12 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name caspase 12 (gene/pseudogene)
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 36-42 kDa, 50 kDa
GenBank Accession NumberNR_000035
Gene Symbol Caspase 12
Gene ID (NCBI) 120329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.


Product Specific Protocols
WB protocol for Caspase 12 antibody 55238-1-APDownload protocol
IHC protocol for Caspase 12 antibody 55238-1-APDownload protocol
IF protocol for Caspase 12 antibody 55238-1-APDownload protocol
IP protocol for Caspase 12 antibody 55238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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