Catalase Antibody

Catalase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 21260-1-AP

Synonyms

CAT, catalase



Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells, rat liver tissue, Raji cells, mouse liver tissue, HT-1080 cells
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The 21260-1-AP immunogen is Fusion Protein Catalase protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species Aspergillus flavus strain, bat, goat, human, Meretrix meritrix, mouse, rat, sheep, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Catalase fusion protein Ag15383
Full Name catalase
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession number BC112219
Gene symbol CAT
Gene ID (NCBI) 847
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.

Protocols

Product Specific Protocols
WB protocol for Catalase antibody 21260-1-APDownload protocol
IHC protocol for Catalase antibody 21260-1-APDownload protocol
IF protocol for Catalase antibody 21260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Rep

The role of cytochrome P450 2E1 on ethanol-mediated oxidative stress and HIV replication in human monocyte-derived macrophages.

Authors - Yuqing Gong
ratWB

Environ Toxicol Pharmacol

Hydrogen sulfide ameliorates disorders in the parafacial respiratory group region of neonatal rats caused by prenatal cigarette smoke exposure via an antioxidative effect.

Authors - Xiang Yan
ratWB

Front Physiol

Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion via FoxO1 Nuclear Translocation in H9c2 Cells.

Authors - Dandan Zhou
ratWB

J Sci Food Agric

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Authors - Zhengxuan Wang
mouseWB

Front Endocrinol (Lausanne)

Exenatide Protects Against Cardiac Dysfunction by Attenuating Oxidative Stress in the Diabetic Mouse Heart.

Authors - Wei Ding
mouseWB

Sci Rep

Plasma exosomes exacerbate alcohol- and acetaminophen-induced toxicity via CYP2E1 pathway.

Authors - Mohammad A Rahman