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Catalase Antibody 35 Publications

Rabbit Polyclonal| Catalog number: 21260-1-AP

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Con: 27 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Catalase Antibody WB Jurkat cells 21260-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 21260-1-AP( Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Catalase Antibody IHC human liver cancer tissue 21260-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Catalase Antibody IHC human liver cancer tissue 21260-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Catalase Antibody IF HepG2 cells 21260-1-AP

Immunofluorescent analysis of HepG2 cells using 21260-1-AP(Catalase antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse, rat, bat, goat, Meretrix meritrix, sheep, swine
Positive WB detected in:
Jurkat cells, HepG2 cells, HeLa cells, rat liver tissue, Raji cells, mouse liver tissue, HT-1080 cells
Positive IHC detected in:
human liver cancer tissue, human hepatocirrhosis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:2000-1:8000
IHC : 1:250-1:1000
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Catalase fusion protein Ag15383
Full name:
catalase
Calculated molecular weight:
60 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC112219
Gene ID (NCBI):
847
Gene symbol
CAT
Synonyms
CAT, catalase

Background

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.


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