Tested Applications
| Positive WB detected in | HeLa cells, rat liver tissue, Raji cells, Jurkat cells, HepG2 cells, L02 cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 164 publications below |
| IHC | See 6 publications below |
| IF | See 11 publications below |
| FC | See 1 publications below |
Product Information
21260-1-AP targets Catalase in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, bovine, goat, sheep, bat, meretrix meretrix |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Catalase fusion protein Ag15383 Predict reactive species |
| Full Name | catalase |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC112219 |
| Gene Symbol | Catalase |
| Gene ID (NCBI) | 847 |
| RRID | AB_10733099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04040 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Catalase antibody 21260-1-AP | Download protocol |
| IHC protocol for Catalase antibody 21260-1-AP | Download protocol |
| IP protocol for Catalase antibody 21260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity | ||
Nat Commun Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery. | ||
Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. | ||
Redox Biol LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis. | ||
ACS Appl Mater Interfaces Extracellular Matrix-Mimicking Hydrogel with Angiogenic and Immunomodulatory Properties Accelerates Healing of Diabetic Wounds by Promoting Autophagy |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Silvia (Verified Customer) (08-11-2022) | It worked well for western blotting.
|
FH Maximilian (Verified Customer) (06-03-2022) | The antibody works fine and detected human catalase at 0.01 and 0.05 µg total protein amount in my blot. Accidental storage time of 24h at RT did not affect performance.
|
FH Maximilian (Verified Customer) (05-27-2022) | Antibody performed well in detection of catalase standard protein (catalase from human erythrocytes) but signal for cell lysates could be better (will increase antibody concentration). Maybe this is due to the storage for 24h at room temperature, which was the result of wrong storage by a colleague, as the package was erroneously delivered to her instead of me.
|
FH Tom (Verified Customer) (08-25-2021) | Membrane blocked in BSA solution prior to incubation with antibody (1:2000). Correct band is clearly visible, but with some background.
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