Catalase Polyclonal antibody

Catalase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

80

Reviews (3)

Cat no : 21260-1-AP

Synonyms

CAT, catalase, Cell and organelle markers, Peroxisome Marker

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Validation Data Gallery

• 

  WB analysis of Jurkat using 21260-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21260-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 21260-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21260-1-AP (CAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21260-1-AP

  Immunofluorescent analysis of HepG2 cells using 21260-1-AP (Catalase antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HeLa using 21260-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Catalase antibody (21260-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Raji cells, rat liver tissue, HepG2 cells, L02 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 78 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 21260-1-AP is Catalase Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bat, bovine, Fungus, goat, Meretrix meretrix, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Catalase fusion protein Ag15383
• Full Name: catalase
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 60 kDa
• GenBank accession number: BC112219
• Gene symbol: CAT
• Gene ID (NCBI): 847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.

Publications

Species	Application	Title
human	WB	Biochem Biophys Rep; The role of cytochrome P450 2E1 on ethanol-mediated oxidative stress and HIV replication in human monocyte-derived macrophages.; Authors - Yuqing Gong
rat	WB	Environ Toxicol Pharmacol; Hydrogen sulfide ameliorates disorders in the parafacial respiratory group region of neonatal rats caused by prenatal cigarette smoke exposure via an antioxidative effect.; Authors - Xiang Yan
rat	WB	Front Physiol; PMID: 30930790; Authors - Dandan Zhou
rat	WB	J Sci Food Agric; l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.; Authors - Zhengxuan Wang
mouse	WB	Front Endocrinol (Lausanne); PMID: 31024445; Authors - Wei Ding
mouse	WB	Sci Rep; Plasma exosomes exacerbate alcohol- and acetaminophen-induced toxicity via CYP2E1 pathway.; Authors - Mohammad A Rahman

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maximilian (Verified Customer) (06-03-2022)

The antibody works fine and detected human catalase at 0.01 and 0.05 µg total protein amount in my blot. Accidental storage time of 24h at RT did not affect performance.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2

FH

Maximilian (Verified Customer) (05-27-2022)

Antibody performed well in detection of catalase standard protein (catalase from human erythrocytes) but signal for cell lysates could be better (will increase antibody concentration). Maybe this is due to the storage for 24h at room temperature, which was the result of wrong storage by a colleague, as the package was erroneously delivered to her instead of me.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2

FH

Tom (Verified Customer) (08-25-2021)

Membrane blocked in BSA solution prior to incubation with antibody (1:2000). Correct band is clearly visible, but with some background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

Catalase Antibody Western Blot validation (1:2000 dilution) in (Cat no:21260-1-AP)

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