Catalase Polyclonal antibody

Catalase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21260-1-AP

Synonyms

CAT, catalase, Cell and organelle markers, Peroxisome Marker



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, Raji cells, rat liver tissue, HepG2 cells, L02 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21260-1-AP is Catalase Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bat, bovine, Fungus, goat, Meretrix meretrix, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Catalase fusion protein Ag15383
Full Name catalase
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC112219
Gene symbol CAT
Gene ID (NCBI) 847
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.

Protocols

Product Specific Protocols
WB protocol for Catalase antibody 21260-1-APDownload protocol
IHC protocol for Catalase antibody 21260-1-APDownload protocol
IF protocol for Catalase antibody 21260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Rep

The role of cytochrome P450 2E1 on ethanol-mediated oxidative stress and HIV replication in human monocyte-derived macrophages.

Authors - Yuqing Gong
ratWB

Environ Toxicol Pharmacol

Hydrogen sulfide ameliorates disorders in the parafacial respiratory group region of neonatal rats caused by prenatal cigarette smoke exposure via an antioxidative effect.

Authors - Xiang Yan
ratWB

Front Physiol

PMID: 30930790

Authors - Dandan Zhou
ratWB

J Sci Food Agric

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Authors - Zhengxuan Wang
mouseWB

Front Endocrinol (Lausanne)

PMID: 31024445

Authors - Wei Ding
mouseWB

Sci Rep

Plasma exosomes exacerbate alcohol- and acetaminophen-induced toxicity via CYP2E1 pathway.

Authors - Mohammad A Rahman

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maximilian (Verified Customer) (06-03-2022)

The antibody works fine and detected human catalase at 0.01 and 0.05 µg total protein amount in my blot. Accidental storage time of 24h at RT did not affect performance.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HepG2
FH

Maximilian (Verified Customer) (05-27-2022)

Antibody performed well in detection of catalase standard protein (catalase from human erythrocytes) but signal for cell lysates could be better (will increase antibody concentration). Maybe this is due to the storage for 24h at room temperature, which was the result of wrong storage by a colleague, as the package was erroneously delivered to her instead of me.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HepG2
FH

Tom (Verified Customer) (08-25-2021)

Membrane blocked in BSA solution prior to incubation with antibody (1:2000). Correct band is clearly visible, but with some background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
Catalase Antibody Western Blot validation (1:2000 dilution) in (Cat no:21260-1-AP)