CATSPER1 Polyclonal antibody

• 

  WB analysis using 12722-1-AP

  human testis lysates were subjected to SDS PAGE followed by western blot with 12722-1-AP (CATSPER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CATSPER1 Polyclonal Antibody for WB, ELISA

Cat No. 12722-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cation channel sperm-associated protein 1, CATSPER, hCatSper

---

Tested Applications

• Positive WB detected in: human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12722-1-AP targets CATSPER1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CATSPER1 fusion protein Ag3417
• Full Name: cation channel, sperm associated 1
• Calculated Molecular Weight: 780 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC032950
• Gene Symbol: CATSPER1
• Gene ID (NCBI): 117144
• RRID: AB_3669148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEC5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cation channel sperm-associated protein 1 (CATSPER1) belongs to a family of four sperm-specific tetrameric voltage-gated cation channels that are highly conserved in humans and mice (PMID: 12402840; 12932298). Definitive evidence that CatSper1 functions as a voltage-gated calcium channel to facilitate hyperactivated sperm motility came from whole-cell patch-clamp measurements of spermatozoa (PMID: 16467839). CATSPER1 channel currents were potentiated in response to intracellular alkalinization, increasing the concentration of intraflagellar calcium and thereby inducing hyperactivity.