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  • KD/KO Validated

CBS Polyclonal antibody

CBS Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14787-1-AP

Synonyms

Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse colon tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue, rat liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14787-1-AP targets CBS in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, human, mouse, Rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBS fusion protein Ag6437
Full Name cystathionine-beta-synthase
Calculated molecular weight 61 kDa
Observed molecular weight 61-63 kDa
GenBank accession numberBC000440
Gene symbol CBS
Gene ID (NCBI) 875
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

Protocols

Product Specific Protocols
WB protocol for CBS antibody 14787-1-APDownload protocol
IHC protocol for CBS antibody 14787-1-APDownload protocol
IF protocol for CBS antibody 14787-1-APDownload protocol
IP protocol for CBS antibody 14787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

3-mercaptopyruvate sulfurtransferase supports endothelial cell angiogenesis and bioenergetics.

Authors - Armita Abdollahi Govar
human,canineWB,IF

Exp Eye Res

Comparative localization of cystathionine beta synthases and cystathionine gamma lyase in canine, non-human primate and human retina.

Authors - Alireza Badiei
humanWB

J Crohns Colitis

Decreased expression of cystathionine β-synthase exacerbates intestinal barrier injury in ulcerative colitis.

Authors - Shanwen Chen
  • KD Validated
mouseWB

Nitric Oxide

Increase of cystathionine-γ-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes.

Authors - Itamar Goren
human

Ann N Y Acad Sci

PMID: 29722030

Authors - Holly M Brown-Borg
mouseWB

Oxid Med Cell Longev

ZYZ-803 Mitigates Endoplasmic Reticulum Stress-Related Necroptosis after Acute Myocardial Infarction through Downregulating the RIP3-CaMKII Signaling Pathway.

Authors - Lingling Chang