|Positive WB detected in||HEK-293 cells, HeLa cells, mouse brain tissue, mouse colon tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue, rat liver tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human pancreas cancer tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14787-1-AP targets CBS in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse, Rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CBS fusion protein Ag6437|
|Calculated molecular weight||61 kDa|
|Observed molecular weight||61-63 kDa|
|GenBank accession number||BC000440|
|Gene ID (NCBI)||875|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).
|Product Specific Protocols|
|WB protocol for CBS antibody 14787-1-AP||Download protocol|
|IHC protocol for CBS antibody 14787-1-AP||Download protocol|
|IF protocol for CBS antibody 14787-1-AP||Download protocol|
|IP protocol for CBS antibody 14787-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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