CCDC157 Polyclonal antibody

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  WB analysis using 32228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32228-1-AP (CCDC157 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CCDC157 Polyclonal Antibody for WB, ELISA

Cat No. 32228-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

KIAA1656, Coiled-coil domain-containing protein 157

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Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32228-1-AP targets CCDC157 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC157 fusion protein Ag37002
• Full Name: coiled-coil domain containing 157
• Calculated Molecular Weight: 84 kDa, 752 aa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC092419
• Gene Symbol: CCDC157
• Gene ID (NCBI): 550631
• RRID: AB_3670225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q569K6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC157 is a member of the coiled‐coil domain‐containing (CCDC) family proteins, wich have a conserved coiled‐coil motif that is responsible for molecular recognition and protein refolding. CCDC157 is highly expressed in the testes from Drosophila to mammals. Bioinformatics database analysis suggests that CCDC157 expression is significantly decreased in NOA patients (PMID: 30741974). An in vitro study has reported that CCDC157 is localized on Golgi membranes along the secretory pathway and interacts with resident proteins of ER‐Golgi membranes (PMID: 31488582).