CCDC69 Polyclonal antibody

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  WB analysis using 31457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31457-1-AP (CCDC69 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 31457-1-AP

  WB result of CCDC69 antibody (31457-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC69 transfected U2OS cells.

• Featured Product
• KD/KO Validated

CCDC69 Polyclonal Antibody for WB, ELISA

Cat No. 31457-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FLJ13705

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, MDA-MB-231 cells, RT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31457-1-AP targets CCDC69 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC69 fusion protein Ag34858
• Full Name: coiled-coil domain containing 69
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC016647
• Gene Symbol: CCDC69
• Gene ID (NCBI): 26112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: A6NI79
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC69 (Coiled-coil domain-containing protein 69), may act as a scaffold to regulate the recruitment and assembly of spindle midzone components, which is required for the localization of AURKB and PLK1 to the spindle midzone (PMID: 20962590). It is highly expressed in duodenum, esophagus, pancreas, prostate, salivary gland, thymus and urinary bladder. The molecular weight of CCDC69 is 34 kDa, and it has been reported that the protein has a molecular weight of 43kDa (PMID: 20962590).