Cyclin A1 Polyclonal antibody

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  WB analysis using 13295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13295-1-AP (Cyclin A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13295-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13295-1-AP (Cyclin A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13295-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13295-1-AP (Cyclin A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cyclin A1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13295-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CCNA1, cyclin A1

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 28 publications below

Product Information

13295-1-AP targets Cyclin A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin A1 fusion protein Ag4005
• Full Name: cyclin A1
• Calculated Molecular Weight: 464 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC036346
• Gene Symbol: Cyclin A1
• Gene ID (NCBI): 8900
• RRID: AB_2071993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78396
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNA1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNA1 binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. CCNA1 was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. In general, the expression of CCNA1 is tissue-specific and high CCNA1 expression is limited to testis; besides, lower levels of CCNA1 expression are also observed in other human cell lines and in healthy brain. Currently, CCNA1 expression has been illustrated to be downregulated in various tumors, including head and neck squamous-cell cancer (HNSCC) and nasopharyngeal carcinoma; and the promoter of the CCNA1 gene is found to be frequently methylated in colon cancer and HNSCC.

Publications

Species	Application	Title
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human	WB	Sci Rep; PMID: 27731405; Authors - Yuan Zhao
human	WB	J Cell Mol Med; NDUFA4L2 in smooth muscle promotes vascular remodeling in hypoxic pulmonary arterial hypertension.; Authors - Yun Liu
human	WB	Mol Cancer; MicroRNA-505 functions as a tumor suppressor in endometrial cancer by targeting TGF-α.; Authors - Shuo Chen