Cyclin A1 Polyclonal antibody

Cyclin A1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 13295-1-AP

Synonyms

CCNA1, cyclin A1

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 13295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13295-1-AP (Cyclin A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13295-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13295-1-AP (Cyclin A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 13295-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13295-1-AP (Cyclin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13295-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13295-1-AP (Cyclin A1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 13295-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13295-1-AP (Cyclin A1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13295-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13295-1-AP (Cyclin A1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13295-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13295-1-AP (Cyclin A1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13295-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13295-1-AP (Cyclin A1 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 18 publications below

Product Information

13295-1-AP targets Cyclin A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin A1 fusion protein Ag4005
• Full Name: cyclin A1
• Calculated molecular weight: 464 aa, 52 kDa
• GenBank accession number: BC036346
• Gene symbol: CCNA1
• Gene ID (NCBI): 8900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNA1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNA1 binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. CCNA1 was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. In general, the expression of CCNA1 is tissue-specific and high CCNA1 expression is limited to testis; besides, lower levels of CCNA1 expression are also observed in other human cell lines and in healthy brain. Currently, CCNA1 expression has been illustrated to be downregulated in various tumors, including head and neck squamous-cell cancer (HNSCC) and nasopharyngeal carcinoma; and the promoter of the CCNA1 gene is found to be frequently methylated in colon cancer and HNSCC.

Publications

Species	Application	Title
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human	WB	Sci Rep; PMID: 27731405; Authors - Yuan Zhao
human	WB	J Cell Mol Med; NDUFA4L2 in smooth muscle promotes vascular remodeling in hypoxic pulmonary arterial hypertension.; Authors - Yun Liu
human	WB	Mol Cancer; MicroRNA-505 functions as a tumor suppressor in endometrial cancer by targeting TGF-α.; Authors - Shuo Chen

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