HeLa cells were subjected to SDS PAGE followed by western blot with 11554-1-AP(Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

human lung tissue were subjected to SDS PAGE followed by western blot with 11554-1-AP(Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 11554-1-AP(Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 11554-1-AP( Cyclin E1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

MCF-7 cells were subjected to SDS PAGE followed by western blot with 11554-1-AP( Cyclin E1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 11554-1-AP( Cyclin E1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11554-1-AP( Cyclin E1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

1X10^6 MCF-7 cells were stained with 0.2ug Cyclin E1 antibody (11554-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.