|Positive WB detected in||HT-29 cells, human lung tissue, mouse heart tissue, NIH/3T3 cells, HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells, human placenta tissue, K-562 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
11554-1-AP targets Cyclin E1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||Artemia sinica, human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin E1 fusion protein Ag2110|
|Full Name||cyclin E1|
|Calculated molecular weight||410 aa, 47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC035498|
|Gene ID (NCBI)||898|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis.
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MicroRNA-107 induces cell cycle arrests by directly targeting cyclin E1 in ovarian cancer.
Short-term stimulation with histone deacetylase inhibitor trichostatin a induces epithelial-mesenchymal transition in nasopharyngeal carcinoma cells without increasing cell invasion ability.
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miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells.
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GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (11-17-2020)
Tested using EDTA plasma on an antibody microarray