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- KD/KO Validated
Cyclin E1 Polyclonal antibody
Cyclin E1 Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IHC, IF, FC,ELISA
Conjugate
Unconjugated
134
Cat no : 11554-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, mouse heart tissue, MCF-7 cells, NIH/3T3 cells, HeLa cells, Jurkat cells, human lung tissue, K-562 cells, HepG2 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 126 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
11554-1-AP targets Cyclin E1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | Artemia, human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cyclin E1 fusion protein Ag2110 |
| Full Name | cyclin E1 |
| Calculated molecular weight | 410 aa, 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC035498 |
| Gene symbol | CCNE1 |
| Gene ID (NCBI) | 898 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin E1 antibody 11554-1-AP | Download protocol |
| FC protocol for Cyclin E1 antibody 11554-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation. | ||
Eur J Med Chem Design, synthesis and biological evaluation of novel uracil derivatives bearing 1, 2, 3-triazole moiety as thymidylate synthase (TS) inhibitors and as potential antitumor drugs. | ||
Biochem Biophys Res Commun MicroRNA-107 induces cell cycle arrests by directly targeting cyclin E1 in ovarian cancer.
| ||
BMC Cancer Short-term stimulation with histone deacetylase inhibitor trichostatin a induces epithelial-mesenchymal transition in nasopharyngeal carcinoma cells without increasing cell invasion ability. | ||
J Cell Mol Med miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells. | ||
J Exp Clin Cancer Res GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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