CCR5 Polyclonal antibody

CCR5 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17476-1-AP

Synonyms

C C chemokine receptor type 5, C C CKR 5, CC CKR 5, CCCKR5, CCR 5, CCR5, CD195, CHEMR13, CKR 5, CKR5, CMKBR5, HIV 1 fusion coreceptor, IDDM22



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17476-1-AP targets CCR5 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, macaque
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCR5 fusion protein Ag11493
Full Name chemokine (C-C motif) receptor 5
Calculated molecular weight 352 aa, 41 kDa
GenBank accession numberBC038398
Gene symbol CCR5
Gene ID (NCBI) 1234
RRIDAB_10895966
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages and is known to be an important co-receptor for macrophage-tropic viruses, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2, and RANTES.

Protocols

Product Specific Protocols
IHC protocol for CCR5 antibody 17476-1-APDownload protocol
FC protocol for CCR5 antibody 17476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
humanIF

Nat Immunol

Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer.

Authors - Di Huang
human

J Biol Chem

Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys197 and by transmembrane protein aptamers that target it for lysosomal degradation.

Authors - Lisa M Petti

Sci Rep

PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.

Authors - Jing Li
human

Retrovirology

CCR5 editing by Staphylococcus aureus Cas9 in human primary CD4+ T cells and hematopoietic stem/progenitor cells promotes HIV-1 resistance and CD4+ T cell enrichment in humanized mice.

Authors - Qiaoqiao Xiao
human

Cell Death Dis

Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis.

Authors - Haixu Xu