Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17476-1-AP targets CCR5 in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, macaque |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11493 Product name: Recombinant human CCR5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 257-352 aa of BC038398 Sequence: LNTFQEFFGLNNCSSSNRLDQAMQVTETLGMTHCCINPIIYAFVGEKFRNYLLVFFQKHIAKRFCKCCSIFQQEAPERASSVYTRSTGEQEISVGL Predict reactive species |
| Full Name | chemokine (C-C motif) receptor 5 |
| Calculated Molecular Weight | 352 aa, 41 kDa |
| GenBank Accession Number | BC038398 |
| Gene Symbol | CCR5 |
| Gene ID (NCBI) | 1234 |
| RRID | AB_10895966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages and is known to be an important co-receptor for macrophage-tropic viruses, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2, and RANTES.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCR5 antibody 17476-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCR5 Polyclonal antibody (Cat# 17476-1-AP) has 16 total citations published in journals including Nature Biotechnology, Nature Immunology, Cell Death & Disease, Journal for ImmunoTherapy of Cancer, Cell Reports, Scientific Reports, Journal of Virology, Journal of Biological Chemistry, Retrovirology, and Clinical Rheumatology. Associated research fields encompass cancer immunology, HIV/SIV virology and gene editing, expansion microscopy, neuropathic pain, hypertension, Sjögren's disease, and inflammatory responses.
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Nat Immunol Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer. | ||
Cell Death Dis Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis. | ||
J Immunother Cancer Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression | ||
Cell Rep An IGF2BP3-dependent metabolic circuit governs macrophage recruitment and immunosuppression in glioblastoma. | ||
Pharmaceuticals (Basel) Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis |
