CCR5 Polyclonal antibody

CCR5 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 17476-1-AP

Synonyms

C C chemokine receptor type 5, C C CKR 5, CC CKR 5, CCCKR5, CCR 5, CCR5, CD195, CHEMR13, CKR 5, CKR5, CMKBR5, HIV 1 fusion coreceptor, IDDM22

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Validation Data Gallery

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  IHC staining of human tonsillitis using 17476-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17476-1-AP (CCR5 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17476-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17476-1-AP (CCR5 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of K-562 using 17476-1-AP

  1X10^6 K-562 cells were stained with 0.2ug CCR5 antibody (17476-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: K-562 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 17476-1-AP is CCR5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, macaque
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCR5 fusion protein Ag11493
• Full Name: chemokine (C-C motif) receptor 5
• Calculated molecular weight: 352 aa, 41 kDa
• GenBank accession number: BC038398
• Gene symbol: CCR5
• Gene ID (NCBI): 1234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2 and RANTES.

Publications

Species	Application	Title
human	IF,WB	Nat Immunol; Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer.; Authors - Di Huang
human		J Biol Chem; Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys197 and by transmembrane protein aptamers that target it for lysosomal degradation.; Authors - Lisa M Petti
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li
human	WB	Retrovirology; CCR5 editing by Staphylococcus aureus Cas9 in human primary CD4+ T cells and hematopoietic stem/progenitor cells promotes HIV-1 resistance and CD4+ T cell enrichment in humanized mice.; Authors - Qiaoqiao Xiao
human	WB	Cell Death Dis; Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis.; Authors - Haixu Xu
rat	WB	Prog Neuropsychopharmacol Biol Psychiatry; PMID: 26190414; Authors - Klaudia Kwiatkowski

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