FITC Anti-Human CD106 (1.G11B1)

CD106 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

FITC Fluorescent Dye

Publications

1

CloneNo.

1.G11B1

Cat no : FITC-65049

Synonyms

VCAM1, VCAM-1, V CAM 1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM

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Validation Data Gallery

• 

  FC experiment of HUVEC using FITC-65049

  1X10^6 TNF-alpha treated HUVEC cells were surface stained with 10 ul FITC Anti-Human CD106 (FITC-65049, Clone: 1.G11B1) (green) or FITC-Mouse IgG1 isotype control (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: HUVEC cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

FITC-65049 targets CD106 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: vascular cell adhesion molecule 1
• Calculated Molecular Weight: 739 aa, 81 kDa
• GenBank Accession Number: BC017276
• Gene Symbol: VCAM-1
• Gene ID (NCBI): 7412
• RRID: AB_2920493
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 498 nm / 526 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publications

Species	Application	Title
human	FC	J Physiol Biochem; Hypoxia-induced CCL2/CCR2 axis in adipose-derived stem cells (ADSCs) promotes angiogenesis by human dermal microvascular endothelial cells (HDMECs) in flap tissues; Authors - Bairong Fang