Anti-Human CD107a / LAMP1 (H4A3)

CD107a / LAMP1 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IF, FC

Conjugate

Unconjugated

CloneNo.

H4A3

Cat no : 65051-1-Ig

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65051-1-Ig targets CD107a / LAMP1 in WB, IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 1
GenBank accession numberBC006345
Gene symbol LAMP1
Gene ID (NCBI) 3916
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
IF protocol for CD107a / LAMP1 antibody 65051-1-IgDownload protocol
FC protocol for CD107a / LAMP1 antibody 65051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyWB,IF

Autophagy

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan
humanIF

Cells

The Degradation of TMEM166 by Autophagy Promotes AMPK Activation to Protect SH-SY5Y Cells Exposed to MPP

Authors - Zhaozhong Liao