Anti-Human CD107a / LAMP1 (H4A3)

CD107a / LAMP1 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (3)

Applications

WB, IF, FC

Conjugate

Unconjugated

Publications

4

CloneNo.

H4A3

Cat no : 65051-1-Ig

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

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Validation Data Gallery

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  IF Staining of HeLa using 65051-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 65051-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 65051-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

65051-1-Ig targets CD107a / LAMP1 in WB, IF, FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human adherent peripheral blood cells
• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006345
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• RRID: AB_2881467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Publications

Species	Application	Title
human,monkey	WB,IF	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan
human	IF	Cells; The Degradation of TMEM166 by Autophagy Promotes AMPK Activation to Protect SH-SY5Y Cells Exposed to MPP; Authors - Zhaozhong Liao
		Cell Rep; A bacteria-derived tetramerized protein ameliorates nonalcoholic steatohepatitis in mice via binding and relocating acetyl-coA carboxylase; Authors - Yan Lin
human,mouse	WB	Drug Resist Updat; NDRG1 overcomes resistance to immunotherapy of pancreatic ductal adenocarcinoma through inhibiting ATG9A-dependent degradation of MHC-1; Authors - Zhiheng Zhang

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C