Anti-Human CD107a / LAMP1 (H4A3)

CD107a / LAMP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Cat No. 65051-1-Ig
Clone No.H4A3

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human and More (1)

Applications

WB, IF/ICC, FC (Intra)

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65051-1-Ig targets CD107a / LAMP1 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, monkey
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells Predict reactive species
Full Name lysosomal-associated membrane protein 1
Calculated Molecular Weight45 kDa
Observed Molecular Weight100-120 kDa
GenBank Accession NumberBC006345
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_2881467
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11279
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody 65051-1-IgDownload protocol
IF protocol for CD107a / LAMP1 antibody 65051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyWB,IF

Autophagy

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan
humanIF

Eur J Pharmacol

Cycloheptylprodigiosin from marine bacterium Spartinivicinus ruber MCCC 1K03745T induces a novel form of cell death characterized by Golgi disruption and enhanced secretion of cathepsin D in Non-small cell lung cancer cell lines

Authors - Xiaosi Lin
humanIF

Autophagy

ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms

Authors - Zhijie Xu
humanIF

Cell Rep

A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma

Authors - Hongli Zhang
humanIF

MAbs

Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycan

Authors - Kaori Mukai
humanIF

Cells

The Degradation of TMEM166 by Autophagy Promotes AMPK Activation to Protect SH-SY5Y Cells Exposed to MPP

Authors - Zhaozhong Liao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Christine (Verified Customer) (10-02-2024)

I randomly tried this antibody for western blotting (because this clone has been shown to work in WB in publications) and it worked beautifully at 1:500 on total lysates of HEK293T cells. Could be diluted much further.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T
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