Tested Applications
| Positive WB detected in | Con A treated rat splenocytes, Con A treated mouse splenocytes |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32621-1-AP targets CD134/OX40 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg2936 Product name: Recombinant Mouse CD134/OX40 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-211 aa of NM_011659.2 Sequence: VTARRLNCVKHTYPSGHKCCRECQPGHGMVSRCDHTRDTLCHPCETGFYNEAVNYDTCKQCTQCNHRSGSELKQNCTPTQDTVCRCRPGTQPRQDSGYKLGVDCVPCPPGHFSPGNNQACKPWTNCTLSGKQTRHPASDSLDAVCEDRSLLATLLWETQRPTFRPTTVQSTTVWPRTSELPSPPTLVTPEGP Predict reactive species |
| Full Name | tumor necrosis factor receptor superfamily, member 4 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | NM_011659.2 |
| Gene Symbol | CD134 |
| Gene ID (NCBI) | 22163 |
| RRID | AB_3742682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P47741 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD134/OX40 antibody 32621-1-AP | Download protocol |
| WB protocol for CD134/OX40 antibody 32621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD134/OX40 Polyclonal antibody (Cat# 32621-1-AP) has 1 total citation. It appears in the International Journal of Oncology (Impact Factor 7.3), where it was used for IHC in mouse samples. The associated research field involves autophagy inhibition, breast cancer, and immunomodulation.
| Species | Application | Title |
|---|---|---|
Int J Oncol Discovery of the late autophagy inhibitor FZU‑0045‑053 and its anti‑breast cancer and immunomodulatory effects. |
