CD300c Polyclonal antibody

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  IHC staining of human tonsillitis using 32141-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32141-1-AP (CD300C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 32141-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32141-1-AP (CD300C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD300c Polyclonal Antibody for IHC, ELISA

Cat No. 32141-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

CMRF35, CMRF 35A, CMRF 35, CLM 6, CD300c molecule

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32141-1-AP targets CD300c in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD300c molecule
• Calculated Molecular Weight: 224 aa, 25 kDa
• GenBank Accession Number: BC022279
• Gene Symbol: CD300C
• Gene ID (NCBI): 10871
• RRID: AB_3670205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q08708
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD300c is a member of the CD300 family of receptors, which are type I transmembrane proteins expressed in both myeloid and lymphoid lineages and modulate immune responses by their stimulatory and inhibitory capabilities (PMID: 30485414). CD300c, also known as CLM-6, CMRF-35 and IgSF16, is composed of an IgV-like extracellular domain, a transmembrane domain and a short cytoplasmic tail. It is expressed on the surface of monocytes, macrophages, dendritic cells, neutrophils, some T and B lymphocytes (PMID: 1349532; 30498497). CD300c has been reported as an activating receptor expressed on monocytes and that it has a potential role in inflammatory responses (PMID: 23571507). It has also been identified as a T cell co-inhibitory molecule (PMID: 30498497).