CD34 Polyclonal antibody
CD34 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 14486-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human gliomas tissue, human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue, human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 10 publications below |
| IHC | See 28 publications below |
| IF | See 6 publications below |
| FC | See 1 publications below |
Product Information
14486-1-AP targets CD34 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, bovine, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD34 fusion protein Ag5887 |
| Full Name | CD34 molecule |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 105-120 kDa |
| GenBank accession number | BC039146 |
| Gene symbol | CD34 |
| Gene ID (NCBI) | 947 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD34 antibody 14486-1-AP | Download protocol |
| IHC protocol for CD34 antibody 14486-1-AP | Download protocol |
| IF protocol for CD34 antibody 14486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction. | ||
Int J Mol Med Molecular interplay between microRNA-130a and PTEN in palmitic acid-mediated impaired function of endothelial progenitor cells: Effects of metformin. | ||
Vasc Cell Effect of the vascular endothelial growth factor expression level on angiopoietin-2-mediated nasopharyngeal carcinoma growth. | ||
In Vitro Cell Dev Biol Anim De novo lipogenesis and desaturation of fatty acids during adipogenesis in bovine adipose-derived mesenchymal stem cells. | ||
Cardiovasc Diagn Ther Kallistatin correlates with inflammation in abdominal aortic aneurysm and suppresses its formation in mice. | ||
Ann Transl Med Increased m6A methylation level is associated with the progression of human abdominal aortic aneurysm. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Madan Kumar (Verified Customer) (07-13-2021) | Rat primary hepatocyte cells were subjected to SDS PAGE and performed western blot analysis with CD34 antibody (Cat log# 14486-1-AP). This antibody works good.
|
