CD34 Polyclonal antibody

CD34 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14486-1-AP

Synonyms

CD34, CD34 molecule



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman tonsillitis tissue, human small intestine tissue, human gliomas tissue, human hysteromyoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14486-1-AP targets CD34 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity cattle, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD34 fusion protein Ag5887
Full Name CD34 molecule
Calculated molecular weight 41 kDa
Observed molecular weight 105-120 kDa
GenBank accession numberBC039146
Gene symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.

Protocols

Product Specific Protocols
WB protocol for CD34 antibody 14486-1-APDownload protocol
IHC protocol for CD34 antibody 14486-1-APDownload protocol
IF protocol for CD34 antibody 14486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Med Rep

Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.

Authors - Jin Zhao

Int J Mol Med

Molecular interplay between microRNA-130a and PTEN in palmitic acid-mediated impaired function of endothelial progenitor cells: Effects of metformin.

Authors - Xuemei Gu
mouseIHC

Vasc Cell

Effect of the vascular endothelial growth factor expression level on angiopoietin-2-mediated nasopharyngeal carcinoma growth.

Authors - Hai-Hong Chen
cattleIF

In Vitro Cell Dev Biol Anim

De novo lipogenesis and desaturation of fatty acids during adipogenesis in bovine adipose-derived mesenchymal stem cells.

Authors - Yongli Yue
mouseIHC

Cardiovasc Diagn Ther

Kallistatin correlates with inflammation in abdominal aortic aneurysm and suppresses its formation in mice.

Authors - Yuchen He
humanIHC

Ann Transl Med

Increased m6A methylation level is associated with the progression of human abdominal aortic aneurysm.

Authors - Yuchen He