CD34 Polyclonal antibody

CD34 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 14486-1-AP


CD34, CD34 molecule

Tested Applications

Positive WB detected inHL-60 cells, TF-1 cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human gliomas tissue, human hysteromyoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human liver cancer tissue, human placenta tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14486-1-AP targets CD34 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rabbit, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD34 fusion protein Ag5887
Full Name CD34 molecule
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).


Product Specific Protocols
WB protocol for CD34 antibody 14486-1-APDownload protocol
IHC protocol for CD34 antibody 14486-1-APDownload protocol
IF protocol for CD34 antibody 14486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Madan Kumar (Verified Customer) (07-13-2021)

Rat primary hepatocyte cells were subjected to SDS PAGE and performed western blot analysis with CD34 antibody (Cat log# 14486-1-AP). This antibody works good.

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Rat primary hepatocyte cells