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CD34 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 14486-1-AP

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Con: 37 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

CD34 Antibody WB HL-60 cells 14486-1-AP

HL-60 cells were subjected to SDS PAGE followed by western blot with 14486-1-AP(CD34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

CD34 Antibody IHC human tonsillitis tissue 14486-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14486-1-AP( CD34 antibody) at dilution of 1:600 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD34 Antibody IHC human tonsillitis tissue 14486-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14486-1-AP( CD34 antibody) at dilution of 1:600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, cattle
Positive WB detected in:
HL-60 cells
Positive IHC detected in:
human tonsillitis tissue, human small intestine tissue, human gliomas tissue, human hysteromyoma tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:300-1:1200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD34 fusion protein Ag5887
Full name:
CD34 molecule
Calculated molecular weight:
41 kDa
Observed molecular weight:
105-120 kDa
GenBank accession number:
BC039146
Gene ID (NCBI):
947
Gene symbol
CD34
Synonyms
CD34, CD34 molecule

Background

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.


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