|Positive WB detected in||HL-60 cells|
|Positive IHC detected in||human tonsillitis tissue, human small intestine tissue, human gliomas tissue, human hysteromyoma tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
14486-1-AP targets CD34 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||cattle, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD34 fusion protein Ag5887|
|Full Name||CD34 molecule|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||105-120 kDa|
|GenBank accession number||BC039146|
|Gene ID (NCBI)||947|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.
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