|Positive WB detected in||mouse small intestine tissue, HeLa cells, Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16668-1-AP targets CD40L/CD154 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD40L/CD154 fusion protein Ag10147|
|Full Name||CD40 ligand|
|Calculated molecular weight||261 aa, 29 kDa|
|Observed molecular weight||29-32 kDa|
|GenBank accession number||BC071754|
|Gene ID (NCBI)||959|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.
|Product Specific Protocols|
|WB protocol for CD40L/CD154 antibody 16668-1-AP||Download protocol|
|IHC protocol for CD40L/CD154 antibody 16668-1-AP||Download protocol|
|IF protocol for CD40L/CD154 antibody 16668-1-AP||Download protocol|
|IP protocol for CD40L/CD154 antibody 16668-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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