Validation Data Gallery
|Positive WB detected in
|mouse small intestine tissue, HeLa cells, Jurkat cells
|Positive IP detected in
|Positive IHC detected in
|human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16668-1-AP targets CD40L/CD154 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|CD40L/CD154 fusion protein Ag10147
|Calculated molecular weight
|261 aa, 29 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.
|Product Specific Protocols
|WB protocol for CD40L/CD154 antibody 16668-1-AP
|IHC protocol for CD40L/CD154 antibody 16668-1-AP
|IF protocol for CD40L/CD154 antibody 16668-1-AP
|IP protocol for CD40L/CD154 antibody 16668-1-AP
|Click here to view our Standard Protocols
Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.
J Mol Cell Cardiol
TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κB and PPAR-γ
Br J Pharmacol
CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.
PKCβII-mediated cross-talk of TRPV1/CB2 modulates the glucocorticoid-induced osteoclast overactivity.
Sci China Life Sci
Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.
YAP1 is essential for osteoclastogenesis through a TEADs-dependent mechanism.