CD40L/CD154 Polyclonal antibody

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  WB analysis of mouse small intestine using 16668-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16668-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16668-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40L; CD154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16668-1-AP

  IP result of anti-CD40L/CD154 (IP:16668-1-AP, 4ug; Detection:16668-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 16668-1-AP

  Immunohistochemical analysis of paraffin-embedded tonsillitis slide using 16668-1-AP (CD40L/CD154 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 16668-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using CD40L/CD154 antibody (16668-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 16668-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using CD40L/CD154 antibody (16668-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD40L/CD154 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 16668-1-AP

Publications(14)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, HeLa cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lung cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 5 publications below

Product Information

16668-1-AP targets CD40L/CD154 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10147

  Product name: Recombinant human CD40L; CD154 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-261 aa of BC071754

  Sequence: MIETYNQTSPRSAATGLPISMKIFMYLLTVFLITQMIGSALFAVYLHRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEETKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

• Full Name: CD40 ligand
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29-39 kDa
• GenBank Accession Number: BC071754
• Gene Symbol: CD40 Ligand
• Gene ID (NCBI): 959
• RRID: AB_2076318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29965
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Sci Adv; Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.; Authors - Liyin Yu
mouse	IHC	J Mol Cell Cardiol; TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κB and PPAR-γ; Authors - Youzhi Zhang
mouse	IHC	Br J Pharmacol; CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.; Authors - Fang Bian
human	WB	Pharmacol Res; PKCβII-mediated cross-talk of TRPV1/CB2 modulates the glucocorticoid-induced osteoclast overactivity.; Authors - Giulia Bellini
rat	IHC	Sci China Life Sci; Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.; Authors - Yue Wang
mouse	WB	Bone; YAP1 is essential for osteoclastogenesis through a TEADs-dependent mechanism.; Authors - Liming Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (10-10-2024)

It worked well in immunofluorence, the staining colocalised with the CD3+ cells (T cells) but not with B cells.

• Applications: Immunofluorescence

CD40L/CD154 Antibody Immunofluorescence validation ( dilution) in (Cat no:16668-1-AP)