CD44 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD44 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

202

Reviews (1)

Cat no : 15675-1-AP

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Validation Data Gallery

• 

  WB analysis of HeLa cells using 15675-1-AP

  WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.

• 

  WB analysis using 15675-1-AP

  MDA-MB-231 (CD44+), HeLa (CD44+), A549 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15675-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 15675-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15675-1-AP

  IP Result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.

• 

  IHC staining of human lung cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon cancer using 15675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using 15675-1-AP (CD44 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15675-1-AP

  1X10^6 HeLa cells were stained with 0.20ug CD44 antibody (15675-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, HeLa cells, HUVEC cells, mouse thymus tissue, A549 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, human colon cancer tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 10 publications below
• WB: See 139 publications below
• IHC: See 50 publications below
• IF: See 47 publications below
• FC: See 7 publications below
• CoIP: See 1 publications below

Product Information

15675-1-AP targets CD44 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Bovine, sika deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD44 fusion protein Ag8143
• Full Name: CD44 molecule (Indian blood group)
• Calculated molecular weight: 742 aa, 82 kDa
• Observed molecular weight: 80-95 kDa
• GenBank accession number: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Publications

Species	Application	Title
	IF	Nature; ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.; Authors - Ting Zhang
human	IHC	J Exp Med; Targeting phospholipase D1 attenuates intestinal tumorigenesis by controlling β-catenin signaling in cancer-initiating cells.; Authors - Dong Woo Kang
human	CoIP	Nat Commun; Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties.; Authors - Masaki Takasugi ; KD Validated
human	IHC	Nat Commun; 5-FU promotes stemness of colorectal cancer via p53-mediated WNT/β-catenin pathway activation.; Authors - Yong-Hee Cho
mouse	WB,IHC,IF	J Clin Invest; circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.; Authors - Zhenzhen Chen
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (06-23-2019)

This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 and 1:200
• Cell Tissue Type: Human cell lines

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