- Featured Product
- KD/KO Validated
CD44 Polyclonal antibody
CD44 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15675-1-AP
Validation Data Gallery
|Positive WB detected in||MDA-MB-231 cells, HeLa cells, HUVEC cells, mouse thymus tissue, A549 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, human colon cancer tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15675-1-AP targets CD44 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Bovine, sika deer|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD44 fusion protein Ag8143|
|Full Name||CD44 molecule (Indian blood group)|
|Calculated molecular weight||742 aa, 82 kDa|
|Observed molecular weight||80-95 kDa|
|GenBank accession number||BC004372|
|Gene ID (NCBI)||960|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
|Product Specific Protocols|
|WB protocol for CD44 antibody 15675-1-AP||Download protocol|
|IHC protocol for CD44 antibody 15675-1-AP||Download protocol|
|IF protocol for CD44 antibody 15675-1-AP||Download protocol|
|IP protocol for CD44 antibody 15675-1-AP||Download protocol|
|FC protocol for CD44 antibody 15675-1-AP||Download protocol|
|Click here to view our Standard Protocols|
ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.
J Exp Med
Targeting phospholipase D1 attenuates intestinal tumorigenesis by controlling β-catenin signaling in cancer-initiating cells.
Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties.
5-FU promotes stemness of colorectal cancer via p53-mediated WNT/β-catenin pathway activation.
J Clin Invest
circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.
Cell Death Differ
CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kishor (Verified Customer) (06-23-2019)
This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.