CD44 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD44 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

181

Reviews (1)

Cat no : 15675-1-AP

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Validation Data Gallery

• 

  WB analysis of HeLa cells using 15675-1-AP

  WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.

• 

  WB analysis using 15675-1-AP

  MDA-MB-231 (CD44+), HeLa (CD44+), A549 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 15675-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15675-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15675-1-AP

  IP Result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.

• 

  IHC staining of human lung cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD44 antibody included
• 

  IHC staining of human lung cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD44 antibody included
• 

  IHC staining of human skin using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15675-1-AP (CD44 antibody) at dilution of 1:50 (under 10x lens).

  Ready-to-use IHC kits available with CD44 antibody included
• 

  IHC staining of human skin using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15675-1-AP (CD44 antibody) at dilution of 1:50 (under 40x lens).

  Ready-to-use IHC kits available with CD44 antibody included
• 

  IHC staining of human colon cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD44 antibody included
• 

  IHC staining of human colon cancer using 15675-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD44 antibody included
• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon cancer using 15675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using 15675-1-AP (CD44 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15675-1-AP

  1X10^6 HeLa cells were stained with 0.20ug CD44 antibody (15675-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, HeLa cells, HUVEC cells, mouse thymus tissue, A549 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human skin tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, human colon cancer tissue
• Positive FC detected in: HeLa cells

Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 10 publications below
• WB: See 126 publications below
• IHC: See 47 publications below
• IF: See 41 publications below
• FC: See 5 publications below
• CoIP: See 1 publications below

Product Information

15675-1-AP targets CD44 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Bovine, sika deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD44 fusion protein Ag8143
• Full Name: CD44 molecule (Indian blood group)
• Calculated molecular weight: 742 aa, 82 kDa
• Observed molecular weight: 80-95 kDa
• GenBank accession number: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Publications

Species	Application	Title
human	IF	Nat Commun; High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.; Authors - Byungjun Kang
human	WB	Biochem Biophys Res Commun; MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway.; Authors - Wang Li ; KD Validated
human	WB	Int J Food Sci Nutr; Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.; Authors - Miaomiao Ge
human	FC	Gene; Long non-coding RNA DILC promotes the progression of gallbladder carcinoma.; Authors - Chi Liang
human	WB	J Cell Physiol; FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.; Authors - Yuling Hong
human	IF	Cancers (Basel); A Therapeutic Strategy for Chemotherapy-Resistant Gastric Cancer via Destabilization of Both β-Catenin and RAS.; Authors - Won-Ji Ryu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (06-23-2019)

This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 and 1:200
• Cell Tissue Type: Human cell lines

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