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CD44 Polyclonal antibody
CD44 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, FC, CoIP,ELISA
Conjugate
Unconjugated
132
Cat no : 15675-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MDA-MB-231 cells, HeLa cells, mouse thymus tissue, A549 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, human colon cancer tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 96 publications below |
| IHC | See 34 publications below |
| IF | See 26 publications below |
| FC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
15675-1-AP targets CD44 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | Bovine, human, mouse, rat, sika deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD44 fusion protein Ag8143 |
| Full Name | CD44 molecule (Indian blood group) |
| Calculated molecular weight | 742 aa, 82 kDa |
| Observed molecular weight | 80-95 kDa |
| GenBank accession number | BC004372 |
| Gene symbol | CD44 |
| Gene ID (NCBI) | 960 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD44 antibody 15675-1-AP | Download protocol |
| IHC protocol for CD44 antibody 15675-1-AP | Download protocol |
| IF protocol for CD44 antibody 15675-1-AP | Download protocol |
| IP protocol for CD44 antibody 15675-1-AP | Download protocol |
| FC protocol for CD44 antibody 15675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy. | ||
Biochem Biophys Res Commun MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway.
| ||
Int J Food Sci Nutr Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway. | ||
J Cell Physiol FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression. | ||
Cancers (Basel) A Therapeutic Strategy for Chemotherapy-Resistant Gastric Cancer via Destabilization of Both β-Catenin and RAS. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (06-23-2019) | This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.
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