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CD44 Antibody 63 Publications

Rabbit Polyclonal| Catalog number: 15675-1-AP

Featured Product KD/KO validated

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

CD44 Antibody WB HeLa cells 15675-1-AP

WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.

CD44 Antibody WB HeLa cells 15675-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15675-1-AP(CD44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CD44 Antibody IF HeLa cells 15675-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CD44 Antibody IHC human lung cancer tissue 15675-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15675-1-AP( CD44 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD44 Antibody IHC human lung cancer tissue 15675-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15675-1-AP( CD44 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD44 Antibody IP HeLa cells 15675-1-AP

IP Result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.

CD44 Antibody FC HeLa cells 15675-1-AP

1X10^6 HeLa cells were stained with 0.2ug CD44 antibody (15675-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488 -Goat anti-Rabbit IgG with dilution 1:100.

Species specificity:
human, mouse, rat, Bovine, sika deer
Positive WB detected in:
HeLa cells, MCF-7 cells, mouse thymus tissue, Raji cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human lung cancer tissue, human colon cancer tissue, human skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells, human colon cancer tissue, MCF-7 cells, HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:100-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD44 fusion protein Ag8143
Full name:
CD44 molecule (Indian blood group)
Calculated molecular weight:
742aa,82 kDa
Observed molecular weight:
80-95 kDa
GenBank accession number:
BC004372
Gene ID (NCBI):
960
Gene symbol
CD44
Synonyms
CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.


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