Anti-Mouse CD55 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98228-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD55 Rabbit Recombinant Antibody (98228-1-RR, Clone: 241808D11) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98228-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD55 Rabbit Recombinant Antibody (98228-1-RR, Clone:241808D11) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

CD55 Recombinant Antibody for FC

Cat No. 98228-1-RR

Clone No.241808D11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

DAF-GPI, Daf1, Daf, Complement decay-accelerating factor, GPI-anchored, Cd55a

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98228-1-RR targets CD55 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD55 antigen
• Calculated Molecular Weight: 43kDa
• GenBank Accession Number: NM_010016.3
• Gene Symbol: CD55
• Gene ID (NCBI): 13136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q61475
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044).