CD59 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD59 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10742-1-AP

Synonyms

16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin

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Validation Data Gallery

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  WB analysis using 10742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10742-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 10742-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 10742-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 10742-1-AP (CD59 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10742-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CD59 (10742-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, human brain tissue, HUVEC cells, HeLa cells
• Positive IHC detected in: human placenta tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: BxPC-3 cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10742-1-AP targets CD59 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD59 fusion protein Ag1173
• Full Name: CD59 molecule, complement regulatory protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC001506
• Gene Symbol: CD59
• Gene ID (NCBI): 966
• RRID: AB_2076228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium; Authors - Philipp Dörschmann
human	WB,IHC	Int J Oncol; The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer.; Authors - Qianwen Ouyang
human	WB	PLoS One; Gecko CD59 is implicated in proximodistal identity during tail regeneration.; Authors - Wang Yongjun Y
	WB	Chem Res Toxicol; 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.; Authors - Mikhail Linetsky
human	WB	Biochem Biophys Rep; Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica.; Authors - S Nishiyama
human	WB,IF	Mol Cell Endocrinol; Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.; Authors - Masashi Ueda ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C