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  • KD/KO Validated

CD59 Polyclonal antibody

CD59 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 10742-1-AP


16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin

Tested Applications

Positive WB detected inBxPC-3 cells, human brain tissue, HUVEC cells, HeLa cells
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10742-1-AP targets CD59 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD59 fusion protein Ag1173
Full Name CD59 molecule, complement regulatory protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC001506
Gene Symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.


Product Specific Protocols
WB protocol for CD59 antibody 10742-1-APDownload protocol
IHC protocol for CD59 antibody 10742-1-APDownload protocol
IF protocol for CD59 antibody 10742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Mol Sci

Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium

Authors - Philipp Dörschmann

Int J Oncol

The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer.

Authors - Qianwen Ouyang

PLoS One

Gecko CD59 is implicated in proximodistal identity during tail regeneration.

Authors - Wang Yongjun Y

Chem Res Toxicol

4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.

Authors - Mikhail Linetsky

Biochem Biophys Rep

Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica.

Authors - S Nishiyama

Mol Cell Endocrinol

Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.

Authors - Masashi Ueda
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Macarena Lucia (Verified Customer) (10-17-2022)

clear band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000