Validation Data Gallery
|Positive WB detected in
|BxPC-3 cells, human brain tissue, HUVEC cells, HeLa cells
|Positive IHC detected in
|human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10742-1-AP targets CD59 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|CD59 fusion protein Ag1173
|CD59 molecule, complement regulatory protein
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Int J Mol Sci
Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium
Int J Oncol
The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer.
Gecko CD59 is implicated in proximodistal identity during tail regeneration.
Chem Res Toxicol
4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.
Biochem Biophys Rep
Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica.
Mol Cell Endocrinol
Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.
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Macarena Lucia (Verified Customer) (10-17-2022)