Validation Data Gallery
|Positive WB detected in||BxPC-3 cells, human brain tissue, HUVEC cells, HeLa cells|
|Positive IHC detected in||human placenta tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||BxPC-3 cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10742-1-AP targets CD59 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD59 fusion protein Ag1173|
|Full Name||CD59 molecule, complement regulatory protein|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||18-20 kDa|
|GenBank accession number||BC001506|
|Gene ID (NCBI)||966|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Int J Oncol
The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer.
Gecko CD59 is implicated in proximodistal identity during tail regeneration.
Chem Res Toxicol
4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.
Biochem Biophys Rep
Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica.
Mol Cell Endocrinol
Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.
Clin Exp Immunol
Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.
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Macarena Lucia (Verified Customer) (10-17-2022)