|Positive WB detected in||BxPC-3 cells, human brain tissue, HUVEC cells, HeLa cells|
|Positive IHC detected in||human tonsillitis tissue, human ovary tumor tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||BxPC-3 cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10742-1-AP targets CD59 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD59 fusion protein Ag1173|
|Full Name||CD59 molecule, complement regulatory protein|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||18-20 kDa|
|GenBank accession number||BC001506|
|Gene ID (NCBI)||966|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
|Product Specific Protocols|
|WB protocol for CD59 antibody 10742-1-AP||Download protocol|
|IHC protocol for CD59 antibody 10742-1-AP||Download protocol|
|IF protocol for CD59 antibody 10742-1-AP||Download protocol|
|FC protocol for CD59 antibody 10742-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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