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  • KD/KO Validated

CD59 Polyclonal antibody

CD59 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10742-1-AP

Synonyms

16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin



Tested Applications

Positive WB detected inBxPC-3 cells, human brain tissue, HUVEC cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue, human ovary tumor tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10742-1-AP targets CD59 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD59 fusion protein Ag1173
Full Name CD59 molecule, complement regulatory protein
Calculated molecular weight 19 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC001506
Gene symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Protocols

Product Specific Protocols
WB protocol for CD59 antibody 10742-1-APDownload protocol
IHC protocol for CD59 antibody 10742-1-APDownload protocol
IF protocol for CD59 antibody 10742-1-APDownload protocol
FC protocol for CD59 antibody 10742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Cell Endocrinol

Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.

Authors - Masashi Ueda
  • KD Validated
mouseWB

Clin Exp Immunol

Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.

Authors - Yingchun Zhang
humanWB

Biochem Biophys Rep

Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica.

Authors - S Nishiyama
WB

Chem Res Toxicol

4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.

Authors - Mikhail Linetsky
humanWB

PLoS One

Gecko CD59 is implicated in proximodistal identity during tail regeneration.

Authors - Wang Yongjun Y