CD63 Polyclonal antibody

CD63 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

Cat no : 25682-1-AP

Synonyms

CD63, CD63 antigen, CD63 molecule, Granulophysin, LAMP 3, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30



Tested Applications

Positive WB detected inhuman serum exosomes
Positive IHC detected inhuman malignant melanoma tissue, human lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25682-1-AP is CD63 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD63 fusion protein Ag19690
Full Name CD63 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC002349
Gene symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD63, known as LAMP-3, is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Protocols

Product Specific Protocols
WB protocol for CD63 antibody 25682-1-APDownload protocol
IHC protocol for CD63 antibody 25682-1-APDownload protocol
IF protocol for CD63 antibody 25682-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Transfer of Extracellular Vesicle-Associated-RNAs Induces Drug Resistance in ALK-Translocated Lung Adenocarcinoma.

Authors - Hoi-Hin Kwok
humanWB

J Biol Chem

Modulation of calreticulin expression reveals a novel exosome mediated mechanism of Z variant alpha-1 antitrypsin disposal.

Authors - Nazli Khodayari
humanWB

Int J Biol Macromol

Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2.

Authors - Hui-Ying Hu
humanWB

Stem Cell Res Ther

Exosomes secreted by mesenchymal stromal/stem cell-derived adipocytes promote breast cancer cell growth via activation of Hippo signaling pathway.

Authors - Shihua Wang
human,mouseWB

Sci Rep

Plasma exosomes exacerbate alcohol- and acetaminophen-induced toxicity via CYP2E1 pathway.

Authors - Mohammad A Rahman

Hepatology

A Noncoding Regulatory RNAs Network Driven by Circ-CDYL Acts Specifically in the Early Stages Hepatocellular Carcinoma.

Authors - Yanping Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (03-30-2020)

Used for both SH-SY5Y lysate and exosomes/ extracellular vesicles. Very good signal in lysates (could be used at much higher dilution). Also good signal in exosomes, which are notoriously difficult to assess by immunoblot. Very impressive results compared to other markers.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: SH-SY5Y