CD63 Polyclonal antibody

CD63 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 25682-1-AP

Synonyms

CD63, CD63 antigen, CD63 molecule, Cell and organelle markers, Endosome Marker, Exosome marker, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30



Tested Applications

Positive WB detected inhuman serum exosomes
Positive IHC detected inhuman malignant melanoma tissue, human tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman malignant melanoma tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25682-1-AP is CD63 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, canine, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD63 fusion protein Ag19690
Full Name CD63 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC002349
Gene symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Protocols

Product Specific Protocols
WB protocol for CD63 antibody 25682-1-APDownload protocol
IHC protocol for CD63 antibody 25682-1-APDownload protocol
IF protocol for CD63 antibody 25682-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
humanWB

Small Methods

Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers.

Authors - Xuehui Liu
rat

Bioact Mater

Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration.

Authors - Lei Fan
humanWB

Theranostics

Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis.

Authors - Yubin Li
humanWB

ACS Appl Mater Interfaces

Beehive-Inspired Macroporous SERS Probe for Cancer Detection through Capturing and Analyzing Exosomes in Plasma.

Authors - Shilian Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nydia (Verified Customer) (06-11-2022)

The HeLa cells as well as Lamp1-expressing stem cells used in the immunostaining were transfected or alternatively transfected by splitting the cells later on the glass coverslips. In order to increase cell spreading and adhesion, the coverslips used were acid washed and treated with fibronectin. Afterwards, the cells were fixed with 4% PFA, permeabilized with 0.2% Triton X-100 in phosphate- buffered saline (PBS) for 10 minutes and for 1 hour the cells were blocked with 5% BSA in PBS. Then overnight, at 4 degrees Celsius, the primary antibodies were added followed by a wash with PBS 3 times.

  • Applications: Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
CD63 Antibody Cell culture validation (1:1000 dilution) in HeLa cells (Cat no:25682-1-AP)
FH

Guorong (Verified Customer) (03-22-2022)

Smears between 30 and 60 kDa was detected

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ARPE-19
CD63 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:25682-1-AP)
FH

David (Verified Customer) (03-30-2020)

Used for both SH-SY5Y lysate and exosomes/ extracellular vesicles. Very good signal in lysates (could be used at much higher dilution). Also good signal in exosomes, which are notoriously difficult to assess by immunoblot. Very impressive results compared to other markers.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: SH-SY5Y