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CD7 Monoclonal antibody

CD7 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4E6

Cat no : 60209-2-Ig

Synonyms

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD7 Monoclonal antibody (60209-2-Ig)

    WB analysis of Jurkat using 60209-2-Ig

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of various lysates using CD7 Monoclonal antibody (60209-2-Ig)

    WB analysis using 60209-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human appendicitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human appendicitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human lymphoma using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of human lymphoma using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of mouse thymus using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD7 Monoclonal antibody (60209-2-Ig)

    IHC staining of mouse thymus using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, HuH-7 cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, mouse thymus tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60209-2-Ig targets CD7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD7 fusion protein Ag1805
Full Name CD7 molecule
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC009293
Gene Symbol CD7
Gene ID (NCBI) 924
RRIDAB_2881355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

Protocols

Product Specific Protocols
WB protocol for CD7 antibody 60209-2-IgDownload protocol
IHC protocol for CD7 antibody 60209-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols