CD81 Polyclonal antibody

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  WB analysis of Raji using 27855-1-AP

  Jurkat cells and Raji cells were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 27855-1-AP

  WB result of CD81 antibody (27855-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells.

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  WB analysis using 27855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27855-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27855-1-AP (CD81 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27855-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27855-1-AP (CD81 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CD81 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27855-1-AP

Publications(88)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

Tetraspanin 28, TAPA1, S5.7, CD81 molecule, CD81 antigen

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Tested Applications

• Positive WB detected in: Raji cells, mouse brain tissue, rat brain tissue, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 81 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

27855-1-AP targets CD81 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD81 fusion protein Ag27298
• Full Name: CD81 molecule
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC002978
• Gene Symbol: CD81
• Gene ID (NCBI): 975
• ENSEMBL Gene ID: ENSG00000110651
• RRID: AB_2880995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P60033
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
human	WB	EMBO J; Extracellular vesicles from Zika virus-infected cells display viral E protein that binds ZIKV-neutralizing antibodies to prevent infection enhancement; Authors - Fanfan Zhao
human	WB	J Control Release; 3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing; Authors - Yuehe Xu
rat	WB	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
rat	WB	Adv Healthc Mater; Dual-Barb Microneedle with JAK/STAT Inhibitor-Loaded Nanovesicles Encapsulation for Tendinopathy; Authors - Minhao Chen
human	WB	Cell Commun Signal; Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease; Authors - Qiqing Huang