CD81 Polyclonal antibody

CD81 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 27855-1-AP

Synonyms

CD81, CD81 antigen, CD81 molecule, Cell and organelle markers, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

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Validation Data Gallery

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  WB analysis of Raji using 27855-1-AP

  Jurkat cells and Raji cells were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below

Product Information

27855-1-AP targets CD81 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD81 fusion protein Ag27298
• Full Name: CD81 molecule
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 23 kDa
• GenBank accession number: BC002978
• Gene symbol: CD81
• Gene ID (NCBI): 975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; KLF4-mediated upregulation of CD9 and CD81 suppresses hepatocellular carcinoma development via JNK signaling.; Authors - Yandong Li ; KD Validated
human	WB	Stem Cells Int; Similar Repair Effects of Human Placenta, Bone Marrow Mesenchymal Stem Cells, and Their Exosomes for Damaged SVOG Ovarian Granulosa Cells.; Authors - Shuwen Chen
human	WB	Cell Death Dis; TGF-β1-mediated exosomal lnc-MMP2-2 increases blood-brain barrier permeability via the miRNA-1207-5p/EPB41L5 axis to promote non-small cell lung cancer brain metastasis.; Authors - Dongming Wu
mouse	WB	Aging (Albany NY); Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis.; Authors - Mei-Ling Xu
rat	WB	Biochem Biophys Res Commun; Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.; Authors - Bao-Cheng Yang
human	WB	Exp Cell Res; Exosomes secreted from mesenchymal stem cells mediate the regeneration of endothelial cells treated with rapamycin by delivering pro-angiogenic microRNAs.; Authors - Weizong Wang