CD81 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD81 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 27855-1-AP

Synonyms

CD81, CD81 antigen, CD81 molecule, Cell and organelle markers, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

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Validation Data Gallery

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  WB analysis of Raji using 27855-1-AP

  Jurkat cells and Raji cells were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 2 publications below

Product Information

27855-1-AP targets CD81 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD81 fusion protein Ag27298
• Full Name: CD81 molecule
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 23 kDa
• GenBank accession number: BC002978
• Gene symbol: CD81
• Gene ID (NCBI): 975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publications

Species	Application	Title
mouse	WB	J Control Release; Engineered exosome as targeted lncRNA MEG3 delivery vehicles for osteosarcoma therapy.; Authors - Xin Huang
human	WB	J Pathol; Inflammatory capacity of exosomes released in the early stages of acute pancreatitis predicts the severity of the disease.; Authors - Montserrat Carrascal
mouse	WB	Front Oncol; iRGD-modified exosomes-delivered BCL6 siRNA inhibit the progression of diffuse large B-cell lymphoma; Authors - Qinhua Liu
	WB	Biomed Pharmacother; Insight into the proteomic profiling of exosomes secreted by human OM-MSCs reveals a new potential therapy.; Authors - Chengfeng Xun
mouse	WB	Int J Biol Sci; Exosomes derived from Wharton's jelly of human umbilical cord mesenchymal stem cells reduce osteocyte apoptosis in glucocorticoid-induced osteonecrosis of the femoral head in rats via the miR-21-PTEN-AKT signalling pathway.; Authors - Ming-Jie Kuang
human	WB	Cardiovasc Drugs Ther; Differential Proteomic Profiles of Coronary Serum Exosomes in Acute Myocardial Infarction Patients with or Without Diabetes Mellitus: ANGPTL6 Accelerates Regeneration of Endothelial Cells Treated with Rapamycin via MAPK Pathways; Authors - Weizong Wang