|Positive WB detected in||Raji cells, Jurkat cells, LPS treated THP-1 cells, Ramos cells, Daudi cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||Jurkat cells, Raji cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:600-1:2400|
|Sample-dependent, check data in validation data gallery|
66866-1-Ig targets CD81 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD81 fusion protein Ag27298|
|Full Name||CD81 molecule|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC002978|
|Gene ID (NCBI)||975|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).
|Product Specific Protocols|
|WB protocol for CD81 antibody 66866-1-Ig||Download protocol|
|IHC protocol for CD81 antibody 66866-1-Ig||Download protocol|
|IF protocol for CD81 antibody 66866-1-Ig||Download protocol|
|FC protocol for CD81 antibody 66866-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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