CD86 Polyclonal antibody

CD86 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13395-1-AP

Synonyms

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, FUN 1, LAB72



Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IF detected inrat brain tissue
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13395-1-AP targets CD86 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD86 fusion protein Ag4063
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC040261
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).

Protocols

Product Specific Protocols
WB protocol for CD86 antibody 13395-1-APDownload protocol
IF protocol for CD86 antibody 13395-1-APDownload protocol
FC protocol for CD86 antibody 13395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Neuropsychiatr Dis Treat

Soluble epoxide hydrolase inhibition enhances anti-inflammatory and antioxidative processes, modulates microglia polarization, and promotes recovery after ischemic stroke.

Authors - Chien-Fu Yeh
humanIHC

Viral Immunol

A Comparison of Splenic Pathologic Change and Immune Function in HBV-Related Portal Hypertension and Chinese Budd-Chiari Syndrome Patients with Hypersplenism.

Authors - Suxin Li
humanIF

Int Immunopharmacol

PMID: 29101873

Authors - Shan Chen
ratWB,IF

Cell Signal

KDM3A inhibition modulates macrophage polarization to aggravate post-MI injuries and accelerates adverse ventricular remodeling via an IRF4 signaling pathway.

Authors - Xiaopei Liu
ratIF

Biomed Pharmacother

Baicalein administered in the subacute phase ameliorates ischemia-reperfusion-induced brain injury by reducing neuroinflammation and neuronal damage.

Authors - Shilun Yang
ratWB

Front Cell Neurosci

Electroacupuncture Pretreatment Alleviates Cerebral Ischemic Injury Through α7 Nicotinic Acetylcholine Receptor-Mediated Phenotypic Conversion of Microglia.

Authors - Zhi Ma