CD8a Polyclonal antibody

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  WB analysis using 33186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33186-1-AP (CD8a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 33186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 33186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat thymus using 33186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 33186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD8a Polyclonal Antibody for WB, IHC, ELISA

Cat No. 33186-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD8, CD8 antigen 32 kDa chain, CD8a molecule, OX-8 membrane antigen, T-cell surface glycoprotein CD8 alpha chain

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Tested Applications

• Positive WB detected in: mouse thymus tissue, rat spleen tissue
• Positive IHC detected in: rat spleen tissue, rat thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33186-1-AP targets CD8a in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD8a fusion protein Eg3839
• Full Name: CD8a molecule
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: NM_031538.2
• Gene Symbol: Cd8a
• Gene ID (NCBI): 24930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P07725
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).