|Positive WB detected in||human placenta tissue, RAW 264.7 cells, L02 cells, mouse placenta tissue, THP-1 cells|
|Positive IHC detected in||human breast cancer tissue, human endometrial cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20597-1-AP is CD9 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||Bat, human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD9 fusion protein Ag14546|
|Full Name||CD9 molecule|
|Calculated molecular weight||228 aa, 25 kDa|
|Observed molecular weight||23-27 kDa|
|GenBank accession number||BC011988|
|Gene ID (NCBI)||928|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
A toolbox for the immunomagnetic purification of signalling organelles.
Int J Biochem Cell Biol
Exosomes from hypoxia-treated human adipose-derived mesenchymal stem cells enhance angiogenesis through VEGF/VEGF-R.
Stem Cell Res Ther
Exosomes from glioma cells induce a tumor-like phenotype in mesenchymal stem cells by activating glycolysis.
CD103-positive CSC exosome promotes EMT of clear cell renal cell carcinoma: role of remote MiR-19b-3p.
Bronchial epithelial cell extracellular vesicles ameliorate epithelial-mesenchymal transition in COPD pathogenesis by alleviating M2 macrophage polarization.
Identification and validation of circulating exosomes-based liquid biopsy for esophageal cancer.