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CD9 Polyclonal antibody

CD9 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 20597-1-AP

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29



Tested Applications

Positive WB detected inHepG2 cells, mouse placenta tissue, RAW 264.7 cells, HeLa cells, L02 cells, human placenta tissue, mouse liver tissue, rat liver tissue, THP-1 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20597-1-AP is CD9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Bat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD9 fusion protein Ag14546
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight 23-30 kDa
GenBank accession numberBC011988
Gene symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

Protocols

Product Specific Protocols
WB protocol for CD9 antibody 20597-1-APDownload protocol
IHC protocol for CD9 antibody 20597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
mouseIHC

Mol Neurodegener

Trem2 deletion enhances tau dispersion and pathology through microglia exosomes

Authors - Bing Zhu
mouseWB

Bioact Mater

Exosome-loaded extracellular matrix-mimic hydrogel with anti-inflammatory property Facilitates/promotes growth plate injury repair.

Authors - Pengfei Guan
rat

Bioact Mater

Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration.

Authors - Lei Fan
humanWB

Theranostics

An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification.

Authors - Shan Xing
humanWB

Theranostics

Exosomes derived from GDNF-modified human adipose mesenchymal stem cells ameliorate peritubular capillary loss in tubulointerstitial fibrosis by activating the SIRT1/eNOS signaling pathway.

Authors - Lu Chen