APC Anti-Human CD95 (DX2)

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  FC experiment of human peripheral blood lymphocytes using APC-65071

  1X10^6 human peripheral blood lymphocytes were surface stained with 10 ul APC-Anti-Human CD95 (APC-65071, clone DX2) (red) or 10 ul APC-mouse IgG1 isotype control (blue). Cells were not fixed.

CD95 Monoclonal Antibody for FC

Cat No. APC-65071

Clone No.DX2

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS1, FASLG receptor, FASTM, TNFRSF6

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Tested Applications

• Positive FC detected in: Human peripheral blood lymphocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-65071 targets CD95 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human CD95 transfected L cells

• Full Name: Fas (TNF receptor superfamily, member 6)
• Calculated Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC012479
• Gene Symbol: Fas/CD95
• Gene ID (NCBI): 355
• RRID: AB_2882971
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P25445
• Storage Buffer: PBS with 0.1% sodium azide and a stabilizer, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.