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  • KD/KO Validated

Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10252-1-AP

Synonyms

CDC20, CDC20A, p55CDC



Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10252-1-AP targets Cdc20 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cdc20 fusion protein Ag0295
Full Name cell division cycle 20 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001088
Gene symbol CDC20
Gene ID (NCBI) 991
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cdc20 antibody 10252-1-APDownload protocol
IHC protocol for Cdc20 antibody 10252-1-APDownload protocol
IP protocol for Cdc20 antibody 10252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Med

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Authors - Yichen Xu
humanWB

Biometals

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Authors - Jieyuan Wu
mouseWB

Cancer Biother Radiopharm

Evaluation of the Antitumor Efficacy of RNAi-Mediated Inhibition of CDC20 and Heparanase in an Orthotopic Liver Tumor Model.

Authors - Meizhou Liu
  • KD Validated
humanWB

Int J Mol Sci

Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.

Authors - Yang Gao
  • KD Validated
mouseWB

bioRxiv

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

Authors - Li Wang
humanWB

Cell Biochem Funct

Anaphase-promoting complex/cyclosome-Cdc-20 promotes Zwint-1 degradation.

Authors - Yan He
  • KD Validated