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  • KD/KO Validated

Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 10252-1-AP


CDC20, CDC20A, p55CDC

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-3 cells, HL-60 cells, Jurkat cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10252-1-AP targets Cdc20 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cdc20 fusion protein Ag0295
Full Name cell division cycle 20 homolog (S. cerevisiae)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001088
Gene Symbol CDC20
Gene ID (NCBI) 991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).


Product Specific Protocols
WB protocol for Cdc20 antibody 10252-1-APDownload protocol
IHC protocol for Cdc20 antibody 10252-1-APDownload protocol
IF protocol for Cdc20 antibody 10252-1-APDownload protocol
IP protocol for Cdc20 antibody 10252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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