Cdc20 Polyclonal antibody

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  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10252-1-AP

  WB result of Cdc20 antibody (10252-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cdc20 transfected HeLa cells.

• 

  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10252-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10252-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10252-1-AP

  IP result of anti-Cdc20 (IP:10252-1-AP, 4ug; Detection:10252-1-AP 1:15000) with Jurkat cells lysate 1400 ug.

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  IHC staining of human breast cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (Cdc20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10252-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cdc20 antibody (10252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Cdc20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10252-1-AP

Publications(46)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

p55CDC, CDC20A

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells, HL-60 cells, Jurkat cells, HepG2 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 37 publications below
• IHC: See 14 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10252-1-AP targets Cdc20 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cdc20 fusion protein Ag0295
• Full Name: cell division cycle 20 homolog (S. cerevisiae)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC001088
• Gene Symbol: CDC20
• Gene ID (NCBI): 991
• RRID: AB_2229016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12834
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Publications

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Sci Signal; Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas; Authors - Matthew J Knarr
human	WB	Elife; Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.; Authors - James Holder
human	WB	Int J Mol Sci; Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.; Authors - Yang Gao ; KD Validated
human	WB	Cell Death Dis; Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.; Authors - Yanlin Huang