- Featured Product
- KD/KO Validated
Cdc20 Polyclonal antibody
Cdc20 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 10252-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
10252-1-AP targets Cdc20 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cdc20 fusion protein Ag0295 |
| Full Name | cell division cycle 20 homolog (S. cerevisiae) |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC001088 |
| Gene symbol | CDC20 |
| Gene ID (NCBI) | 991 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cdc20 antibody 10252-1-AP | Download protocol |
| IHC protocol for Cdc20 antibody 10252-1-AP | Download protocol |
| IP protocol for Cdc20 antibody 10252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Med Translation control of the immune checkpoint in cancer and its therapeutic targeting. | ||
Biometals Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex. | ||
Cancer Biother Radiopharm Evaluation of the Antitumor Efficacy of RNAi-Mediated Inhibition of CDC20 and Heparanase in an Orthotopic Liver Tumor Model.
| ||
Int J Mol Sci Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.
| ||
bioRxiv Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics. | ||
Cell Biochem Funct Anaphase-promoting complex/cyclosome-Cdc-20 promotes Zwint-1 degradation.
|
