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  • KD/KO Validated

Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10252-1-AP

Synonyms

CDC20, CDC20A, p55CDC



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-3 cells, HL-60 cells, Jurkat cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10252-1-AP targets Cdc20 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cdc20 fusion protein Ag0295
Full Name cell division cycle 20 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001088
Gene symbol CDC20
Gene ID (NCBI) 991
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Protocols

Product Specific Protocols
WB protocol for Cdc20 antibody 10252-1-APDownload protocol
IHC protocol for Cdc20 antibody 10252-1-APDownload protocol
IP protocol for Cdc20 antibody 10252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet
humanWB

Elife

Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.

Authors - James Holder
humanWB

Cell Death Dis

Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.

Authors - Yanlin Huang
humanWB

Int J Biol Sci

CDC20 regulates the cell proliferation and radiosensitivity of P53 mutant HCC cells through the Bcl-2/Bax pathway.

Authors - Shuai Zhao
humanWB

Front Oncol

The Oncogenic Role of APC/C Activator Protein Cdc20 by an Integrated Pan-Cancer Analysis in Human Tumors.

Authors - Fei Wu
  • KD Validated
humanWB

iScience

TORC1 inactivation promotes APC/C-dependent mitotic slippage in yeast and human cells.

Authors - Chihiro Yamada