|Positive WB detected in||Jurkat cells, HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10252-1-AP targets Cdc20 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cdc20 fusion protein Ag0295|
|Full Name||cell division cycle 20 homolog (S. cerevisiae)|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC001088|
|Gene ID (NCBI)||991|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
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