CDC25A Recombinant antibody, PBS Only

• 

  WB analysis using 84145-1-RR (same clone as 84145-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84145-1-RR (CDC25A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84145-1-PBS in a different storage buffer formulation.

CDC25A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84145-1-PBS

Clone No.240813F11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

240813F11, CDC25, CDC25A2, EC:3.1.3.48, M-phase inducer phosphatase 1

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Product Information

84145-1-PBS targets CDC25A in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cell division cycle 25 homolog A (S. pombe)
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: NM_001789
• Gene Symbol: CDC25A
• Gene ID (NCBI): 993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P30304
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.