Validation Data Gallery
|Positive WB detected in
|HL-60 cells, HeLa cells, K-562 cells
|Positive IP detected in
|Positive IHC detected in
|human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|HeLa cells, HepG2 cells
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:200-1:800
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
22060-1-AP targets CDK2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|CDK2 fusion protein Ag17133
|cyclin-dependent kinase 2
|Calculated molecular weight
|298 aa, 34 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily. It is dispensable for myelination but is important for adult oligodendrocyte progenitor cell renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin(PMID:21502361). G2 phase CCNA1/CDK2 controls the timing of entry into mitosis by controlling the subsequent activation of CCNB/CDK1, but also has an unexpected role in coordinating the activation of CCNB/CDK1 at the centrosome and in the nucleus(PMID:18372919). It has 2 isoforms produced by alternative splicing with the molecular weight of 34 kDa and 30 kDa.
|Product Specific Protocols
|WB protocol for CDK2 antibody 22060-1-AP
|IHC protocol for CDK2 antibody 22060-1-AP
|IF protocol for CDK2 antibody 22060-1-AP
|IP protocol for CDK2 antibody 22060-1-AP
|Click here to view our Standard Protocols
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Long noncoding RNA HOXC-AS3 interacts with CDK2 to promote proliferation in hepatocellular carcinoma
Eur J Pharmacol
Hypericin-mediated photodynamic therapy inhibits growth of colorectal cancer cells via inducing S phase cell cycle arrest and apoptosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (09-27-2019)
Used in an antibody array to identify proteins in human EDTA plasma.