CDK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 22060-1-AP

Synonyms

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

---

Validation Data Gallery

• 

  WB analysis using 22060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22060-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22060-1-AP

  WB result of CDK2 antibody (22060-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and CDK2 knockout HeLa cells.

• 

  WB analysis using 22060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22060-1-AP (CDK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22060-1-AP

  IP result of anti-CDK2 (IP:22060-1-AP, 4ug; Detection:22060-1-AP 1:1000) with HeLa cells lysate 1200 ug.

• 

  IHC staining of human cervical cancer using 22060-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22060-1-AP (CDK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 22060-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22060-1-AP (CDK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (22060-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 22060-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (22060-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HepG2 using 22060-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (22060-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below

Product Information

22060-1-AP targets CDK2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK2 fusion protein Ag17133
• Full Name: cyclin-dependent kinase 2
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC003065
• Gene Symbol: CDK2
• Gene ID (NCBI): 1017
• RRID: AB_2878982
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily. It is dispensable for myelination but is important for adult oligodendrocyte progenitor cell renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin(PMID:21502361). G2 phase CCNA1/CDK2 controls the timing of entry into mitosis by controlling the subsequent activation of CCNB/CDK1, but also has an unexpected role in coordinating the activation of CCNB/CDK1 at the centrosome and in the nucleus(PMID:18372919). It has 2 isoforms produced by alternative splicing with the molecular weight of 34 kDa and 30 kDa.

Publications

Species	Application	Title
human	IF	Elife; Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.; Authors - Andrew Kodani
human,mouse	WB,IF	Biomark Res; Long noncoding RNA HOXC-AS3 interacts with CDK2 to promote proliferation in hepatocellular carcinoma; Authors - Chen Su
human	IF	Eur J Pharmacol; Hypericin-mediated photodynamic therapy inhibits growth of colorectal cancer cells via inducing S phase cell cycle arrest and apoptosis.; Authors - Jinhang Hu
rat	WB	Front Pharmacol; Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres; Authors - Shaomin Wang
human	WB	Am J Cancer Res; E2F1-mediated GINS2 transcriptional activation promotes tumor progression through PI3K/AKT/mTOR pathway in hepatocellular carcinoma; Authors - Yao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,