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  • KD/KO Validated

CEBPB Polyclonal antibody

CEBPB Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 23431-1-AP

Synonyms

C/EBP beta, CEBPB, CRP2, IL6DBP, LAP, Liver activator protein, NF IL6, Nuclear factor NF IL6, TCF 5, TCF5, Transcription factor 5



Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23431-1-AP is CEBPB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEBPB fusion protein Ag20073
Full Name CCAAT/enhancer binding protein (C/EBP), beta
Calculated molecular weight 345 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC007538
Gene symbol CEBPB
Gene ID (NCBI) 1051
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298). This antibody is specific to CEBPB.

Protocols

Product Specific Protocols
WB protocol for CEBPB antibody 23431-1-APDownload protocol
IHC protocol for CEBPB antibody 23431-1-APDownload protocol
IF protocol for CEBPB antibody 23431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Targeting JUN, CEBPB, and HDAC3: A Novel Strategy to Overcome Drug Resistance in Hypoxic Glioblastoma.

Authors - Yixing Gao
humanWB,chIP

Biochim Biophys Acta Gene Regul Mech

The chromatin remodeling protein BRM regulates the transcription of tight junction proteins: Implication in breast cancer metastasis.

Authors - Yuyu Yang
  • KD Validated
mouseWB

Mol Immunol

Involvement of multiple transcription factors in regulation of IL-β-induced MCP-1 expression in alveolar type II epithelial cells.

Authors - Chunguang Yan

Mol Med Rep

Expression of S100A8 is induced by interleukin‑1α in TR146 epithelial cells through a mechanism involving CCAAT/enhancer binding protein β.

Authors - Mingqun Qin
humanWB, ChIP, RIP

Brain Res Bull

LINC02381 contributes to cell proliferation and hinders cell apoptosis in glioma by transcriptionally enhancing CBX5.

Authors - Yong Sun
mouseWB

Ecotoxicol Environ Saf

Effect of Notch pathway on lipid accumulation induced by mono-2-ethylhexyl phthalate on 3T3-L1 cells.

Authors - Wen Qi