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  • KD/KO Validated

CEBPB Polyclonal antibody

CEBPB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 23431-1-AP

Synonyms

C/EBP beta, CEBPB, CRP2, IL6DBP, LAP, Liver activator protein, NF IL6, Nuclear factor NF IL6, TCF 5, TCF5, Transcription factor 5



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23431-1-AP targets CEBPB in WB, IHC, IF/ICC, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEBPB fusion protein Ag20073
Full Name CCAAT/enhancer binding protein (C/EBP), beta
Calculated Molecular Weight 345 aa, 36 kDa
Observed Molecular Weight42 kDa,46 kDa
GenBank Accession NumberBC007538
Gene Symbol CEBPB
Gene ID (NCBI) 1051
RRIDAB_2879278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298). This antibody is specific to CEBPB.

Protocols

Product Specific Protocols
WB protocol for CEBPB antibody 23431-1-APDownload protocol
IHC protocol for CEBPB antibody 23431-1-APDownload protocol
IF protocol for CEBPB antibody 23431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratCoIP,WB

Adv Sci (Weinh)

HIPK1 Inhibition Protects against Pathological Cardiac Hypertrophy by Inhibiting the CREB-C/EBPβ Axis

Authors - Yihua Bei
humanWB,CoIP

J Immunother Cancer

YY1 complex in M2 macrophage promotes prostate cancer progression by upregulating IL-6

Authors - Saisai Chen
humanWB

Aging (Albany NY)

Functional rare variant in a C/EBP beta binding site in NINJ2 gene increases the risk of coronary artery disease.

Authors - Pengyun Wang
  • KD Validated
humanWB

Int J Mol Sci

ERK1/2-CEBPB Axis-Regulated hBD1 Enhances Anti-Tuberculosis Capacity in Alveolar Type II Epithelial Cells

Authors - Yaoxin Chen
mouseWB

Am J Physiol Cell Physiol

The paracrine isthmin1 transcriptionally regulated by C/EBPβ exacerbates pulmonary vascular leakage in murine sepsis

Authors - Junxia Li

Sci Rep

Identification of a 5-Methylcytosine Site that may Regulate C/EBPβ Binding and Determine Tissue-Specific Expression of the BPI Gene in Piglets.

Authors - Li Sun