CEBPB Polyclonal antibody

• Featured Product
• KD/KO Validated

CEBPB Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 23431-1-AP

Synonyms

C/EBP beta, CEBPB, CRP2, IL6DBP, LAP, Liver activator protein, NF IL6, Nuclear factor NF IL6, TCF 5, TCF5, Transcription factor 5

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 23431-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23431-1-AP (CEBPB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 23431-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23431-1-AP (CEBPB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23431-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23431-1-AP (CEBPB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23431-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23431-1-AP (CEBPB antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23431-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23431-1-AP (CEBPB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 23431-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 23431-1-AP (CEBPB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 23431-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CEBPB antibody (23431-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: RAW 264.7 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IF: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 5 publications below
• RIP: See 1 publications below

Product Information

The immunogen of 23431-1-AP is CEBPB Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEBPB fusion protein Ag20073
• Full Name: CCAAT/enhancer binding protein (C/EBP), beta
• Calculated molecular weight: 345 aa, 36 kDa
• Observed molecular weight: 36 kDa
• GenBank accession number: BC007538
• Gene symbol: CEBPB
• Gene ID (NCBI): 1051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298). This antibody is specific to CEBPB.

Publications

Species	Application	Title
mouse,rat	CoIP,WB	Adv Sci (Weinh); HIPK1 Inhibition Protects against Pathological Cardiac Hypertrophy by Inhibiting the CREB-C/EBPβ Axis; Authors - Yihua Bei
human	WB,CoIP	J Immunother Cancer; YY1 complex in M2 macrophage promotes prostate cancer progression by upregulating IL-6; Authors - Saisai Chen
human	WB	Aging (Albany NY); Functional rare variant in a C/EBP beta binding site in NINJ2 gene increases the risk of coronary artery disease.; Authors - Pengyun Wang ; KD Validated
		Sci Rep; Identification of a 5-Methylcytosine Site that may Regulate C/EBPβ Binding and Determine Tissue-Specific Expression of the BPI Gene in Piglets.; Authors - Li Sun
mouse	WB	Ecotoxicol Environ Saf; Effect of Notch pathway on lipid accumulation induced by mono-2-ethylhexyl phthalate on 3T3-L1 cells.; Authors - Wen Qi
mouse	WB,IF	Environ Toxicol; JMJD3 downregulates IL4i1 aggravating lipopolysaccharide-induced acute lung injury via H3K27 and H3K4 demethylation; Authors - Yizhuo Gao