CEP110 Polyclonal antibody

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• CEP110 Polyclonal antibody (25235-1-AP)

CEP110 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25235-1-AP Copied!

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, ELISA

Synonyms

Centriolin, 110 kDa centrosomal protein, Centrosomal protein 1, centrosomal protein 110kDa, Centrosomal protein of 110 kDa

• 

  WB analysis of Jurkat using 25235-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25235-1-AP (CEP110 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MDCK using 25235-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 25235-1-AP (CEP110 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25235-1-AP targets CEP110 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18907

  Product name: Recombinant human CEP110 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1996-2325 aa of BC137284

  Sequence: VLAAEERVRTLQEEERWCESLEKTLSQTKRQLSEREQQLVEKSGELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLKEALKIQRSQLEKNLLEQKQENSCIQKEMATIELVAQDNHERARRLMKELNQMQYEYTELKKQMANQKDLERRQMEISDAMRTLKSEVKDEIRTSLKNLNQFLPELPADLEAILERNENLEGELESLKENLPFTMNEGPFEEKLNFSQVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLIKGKRQTEGTLHSLRRQVDALGELVTSTSADSASSPSLSQLESSLTEDSQLGQNQEKNASAR

• Full Name: centrosomal protein 110kDa
• Calculated Molecular Weight: 2325 aa, 269 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: BC137284
• Gene Symbol: CEP110
• Gene ID (NCBI): 11064
• RRID: AB_2879979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z7A1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

One reviewer (Jay Kim, 2019) rated this antibody 4/5 stars. They used it at a 1:100 dilution for immunofluorescence on mouse NIH3T3 cells with methanol fixation. The first IF result showed Cep110 localizing at the centrosome, with gamma-Tubulin as a centrosome marker and DAPI for nuclear staining. Overall, the antibody performed well out of the box for IF applications in mouse cells.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Jay (Verified Customer) (10-28-2019)

Methanol fixation.The first IF result showing that cep110 localizes at centrosome in mouse NIH3T3 cell line(Red;Cep110, Green;gamma-Tubulin for centrosome marker, Blue;DAPI for nucleus.)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse NIH3T3 cell line

CEP110 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 cell line (Cat no:25235-1-AP)