CEP97 Polyclonal antibody

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  WB analysis of HL-60 using 22050-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22050-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 22050-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22050-1-AP

  IP result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human breast cancer using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MDCK using 22050-1-AP

  Immunofluorescent analysis of MDCK cells using 22050-1-AP (CEP97 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CEP97 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22050-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

2810403B08Rik, centrosomal protein 97kDa, Centrosomal protein of 97 kDa, CEP97, LRRIQ2

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Tested Applications

• Positive WB detected in: HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IF: See 17 publications below
• IP: See 2 publications below

Product Information

22050-1-AP targets CEP97 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, Canine samples.

• Tested Reactivity: human, Canine
• Cited Reactivity: human, mouse, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16995

  Product name: Recombinant human CEP97 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 506-865 aa of BC041085

  Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV

• Full Name: centrosomal protein 97kDa
• Calculated Molecular Weight: 865 aa, 97 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC041085
• Gene Symbol: CEP97
• Gene ID (NCBI): 79598
• RRID: AB_11182378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IW35
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human,mouse	WB,IF	Nat Commun; M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.; Authors - Ning Huang
human	WB	J Cell Biol; Centrin2 regulates CP110 removal in primary cilium formation.; Authors - Suzanna L Prosser
Drosophila	IF,WB	Curr Biol; Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila.; Authors - Jeroen Dobbelaere
mouse	IF	Curr Biol; Mother Centriole Distal Appendages Mediate Centrosome Docking at the Immunological Synapse and Reveal Mechanistic Parallels with Ciliogenesis.; Authors - Jane C Stinchcombe
human	WB,IF	Am J Hum Genet; Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome.; Authors - Laura Bozal-Basterra