CFTR Polyclonal antibody

CFTR Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20738-1-AP

Synonyms

ABC35, ABCC7, CF, CFTR, CFTR/MRP, MRP7, TNR CFTR



Tested Applications

Positive IHC detected inhuman breast cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20738-1-AP targets CFTR in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Calculated molecular weight 168 kDa
GenBank accession numberNM_000492
Gene symbol CFTR
Gene ID (NCBI) 1080
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

      What is the human cytogenetic location of the CFTR gene?

7q31.2

 

What is the predicted molecular weight of CFTR? 

168 kDa

 

Where is CFTR expressed?

CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.

 

What is the ion selectivity of CFTR?

CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.

 

What is the process of CFTR trafficking?

The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.

 

What are the separate domains of CFTR?

CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.

 

What regulates CFTR?

CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.

 

What is the role of CFTR?

CFTR functions as a channel for anions, regulating water and ionic homeostasis.

 

What mutations in CFTR leads to protein degradation?

Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.

 

How does CFTR differ from other ATP-binding cassette proteins?

Unlike other proteins in the ABC family, CFTR functions as an ion channel.


PMIDs: 24513531, 24534272, 22966013, 22709980


Protocols

Product Specific Protocols
IHC protocol for CFTR antibody 20738-1-APDownload protocol
IF protocol for CFTR antibody 20738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

PLoS One

Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet.

Authors - Mauricio Di Fulvio
mouseWB,IF

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang