CHAT Polyclonal antibody

CHAT Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20747-1-AP


CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2

Tested Applications

Positive WB detected inmouse brain tissue, mouse placenta tissue, Jurkat cells, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse cerebellum tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20747-1-AP targets CHAT in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name choline acetyltransferase
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 47-50 kDa, 66-70 kDa
GenBank Accession NumberNM_020549
Gene Symbol CHAT
Gene ID (NCBI) 1103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively. This antibody can recognize both pCHAT and cCHAT.


Product Specific Protocols
WB protocol for CHAT antibody 20747-1-APDownload protocol
IHC protocol for CHAT antibody 20747-1-APDownload protocol
IF protocol for CHAT antibody 20747-1-APDownload protocol
IP protocol for CHAT antibody 20747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Jerome (Verified Customer) (07-20-2022)

we followed this protocol : antigen retrieval buffer: citrate pH 6 (DAKO S1699) wash in TBS 1X Blocking buffer TBS1x +Serum Goat 5%, 1H at RT Primary and secondary antibody diluted in TBS1X Wash primary antibody TBS1X 0.2%tween Secondary antibody GAR 594 ref A11012 Wash secondary antibody TBS1X 0.2%tween Unfortunately, it doesn't work under these conditions.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Mouse spinal cord

Xin (Verified Customer) (01-23-2022)

Good performance in WB (around 70 kd).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
CHAT Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:20747-1-AP)