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CHD4 Antibody

CHD4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14173-1-AP

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14173-1-AP targets CHD4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHD4 fusion protein Ag5372
Full Name chromodomain helicase DNA binding protein 4
Calculated molecular weight 1937aa,221 kDa
Observed molecular weight 260 kDa
GenBank accession number BC038596
Gene symbol CHD4
Gene ID (NCBI) 1108
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

Protocols

Product Specific Protocols
WB protocol for CHD4 antibody 14173-1-APDownload protocol
IHC protocol for CHD4 antibody 14173-1-APDownload protocol
IF protocol for CHD4 antibody 14173-1-APDownload protocol
IP protocol for CHD4 antibody 14173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Clin Epigenetics

CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.

Authors - Simin Shao
  • KD Validated

Leukemia

Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development.

Authors - B A Vishwakarma