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  • KD/KO Validated

CHD4 Polyclonal antibody

CHD4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14173-1-AP

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14173-1-AP targets CHD4 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHD4 fusion protein Ag5372
Full Name chromodomain helicase DNA binding protein 4
Calculated Molecular Weight 1937 aa, 221 kDa
Observed Molecular Weight 260 kDa
GenBank Accession NumberBC038596
Gene Symbol CHD4
Gene ID (NCBI) 1108
RRIDAB_2080046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

Protocols

Product Specific Protocols
WB protocol for CHD4 antibody 14173-1-APDownload protocol
IHC protocol for CHD4 antibody 14173-1-APDownload protocol
IF protocol for CHD4 antibody 14173-1-APDownload protocol
IP protocol for CHD4 antibody 14173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.

Authors - Xianjiang Lan

Leukemia

Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development.

Authors - B A Vishwakarma
humanChIP,CoIP

Cancer Res

CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors.

Authors - Yijie Wang
  • KD Validated
humanWB,ChIP

Arthritis Rheumatol

Functional Genomic Analysis of a RUNX3 Polymorphism Associated with Ankylosing Spondylitis.

Authors - Matteo Vecellio
humanWB,IF

Clin Epigenetics

CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.

Authors - Simin Shao
  • KD Validated
humanWB,IHC

Cell Signal

CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer

Authors - Jun Zhang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaochen (Verified Customer) (07-31-2023)

It is very sensitive and accurate. I can get the sepecific bend easily.

  • Applications: Western Blot
CHD4 Antibody Western Blot validation ( dilution) in (Cat no:14173-1-AP)
FH

Christos (Verified Customer) (01-26-2023)

Application: IF Sample: Human Cell line (HeLa Kyoto) Fixation: Paraformaldehyde (PFA 4% in PHEM pH=6.9) at room temperature for 15 minutes Permeabilization: 0.1% Triton-X-100 in PBS at room temperature for 5 minutes Blocking step: BSA as blocking agent for 1 hour · Concentration: 5% BSA in PBS · Temperature: RT dilution of the primary antibody: 1/50 incubation time: overnight at 4°C DNA (blue), CHD4 (green) and α-tubulin (red)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: Human cervix carcinoma cells/ HeLe Kyoto
CHD4 Antibody Immunofluorescence validation (1/50 dilution) in Human cervix carcinoma cells/ HeLe Kyoto (Cat no:14173-1-AP)