Chk1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Chk1 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 10362-1-AP

Synonyms

CHEK1, Chk1

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Validation Data Gallery

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  WB analysis of mouse thymus using 10362-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10362-1-AP

  Immunofluorescent analysis of HepG2 cells using 10362-1-AP (Chk1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HepG2 using 10362-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug CHK1 antibody (10362-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse thymus tissue, HeLa cells, K-562 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below

Product Information

10362-1-AP targets Chk1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Chk1 fusion protein Ag0409
• Full Name: CHK1 checkpoint homolog (S. pombe)
• Calculated molecular weight: 54 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC004202
• Gene symbol: CHEK1
• Gene ID (NCBI): 1111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated(PMID:22941630) and ubiquitinated (PMID:19276361). It has 3 isoforms produced by alternative splicing with the molecular weight of 54 kDa, 44 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB	Biometals; Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.; Authors - Jieyuan Wu
rat	WB	Hum Exp Toxicol; Role of inhibiting Chk1-p53 pathway in hepatotoxicity caused by chronic arsenic exposure from coal-burning.; Authors - Yuan Yang
mouse	WB	Plant Foods Hum Nutr; Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.; Authors - Jie Wei
human	IHC	Cancer Chemother Pharmacol; PMID: 32743678; Authors - Jiahong Jiang
mouse	WB	J Agric Food Chem; Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.; Authors - Jie Wei
human	WB	Chem Res Toxicol; PMID: 26451628; Authors - Xiufang Song