Chk1 Polyclonal antibody

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  WB analysis of mouse thymus using 10362-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10362-1-AP (CHK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10362-1-AP

  Immunofluorescent analysis of HepG2 cells using 10362-1-AP (Chk1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• Featured Product
• KD/KO Validated

Chk1 Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 10362-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

CHEK1, Chk1

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Tested Applications

• Positive WB detected in: mouse thymus tissue, HeLa cells, K-562 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 1 publications below

Product Information

10362-1-AP targets Chk1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Chk1 fusion protein Ag0409
• Full Name: CHK1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC004202
• Gene Symbol: Chk1
• Gene ID (NCBI): 1111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14757
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated(PMID:22941630) and ubiquitinated (PMID:19276361). It has 3 isoforms produced by alternative splicing with the molecular weight of 54 kDa, 44 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons.; Authors - Andrew Best ; KD Validated
	WB	Proc Natl Acad Sci U S A; Checkpoint kinase 1 (Chk1)-short is a splice variant and endogenous inhibitor of Chk1 that regulates cell cycle and DNA damage checkpoints.; Authors - Navjotsingh Pabla
human	WB	Oncotarget; UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.; Authors - Xiaojia Gao
human	WB	EBioMedicine; High PARP-1 expression predicts poor survival in acute myeloid leukemia and PARP-1 inhibitor and SAHA-bendamustine hybrid inhibitor combination treatment synergistically enhances anti-tumor effects.; Authors - Xia Li
human	WB	Cell Death Dis; HBV infection potentiates resistance to S-phase arrest-inducing chemotherapeutics by inhibiting CHK2 pathway in diffuse large B-cell lymphoma.; Authors - Xinying Zhao
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi