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Chk1 Polyclonal antibody

Chk1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10362-1-AP

Synonyms

CHEK1, Chk1



Tested Applications

Positive WB detected inmouse thymus tissue, HeLa cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10362-1-AP targets Chk1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chk1 fusion protein Ag0409
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC004202
Gene symbol CHEK1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated(PMID:22941630) and ubiquitinated (PMID:19276361). It has 3 isoforms produced by alternative splicing with the molecular weight of 54 kDa, 44 kDa and 50 kDa.

Publications

SpeciesApplicationTitle
humanWB

Biometals

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Authors - Jieyuan Wu
mouseWB

Plant Foods Hum Nutr

Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.

Authors - Jie Wei
humanIHC

Cancer Chemother Pharmacol

PMID: 32743678

Authors - Jiahong Jiang
mouseWB

J Agric Food Chem

Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.

Authors - Jie Wei
humanWB

Chem Res Toxicol

PMID: 26451628

Authors - Xiufang Song
human

Cancer Biol Ther

Synergistic anti-leukemic interactions between panobinostat and MK-1775 in acute myeloid leukemia ex vivo.

Authors - Wenxiu Qi