HeLa cells were subjected to SDS PAGE followed by western blot with 10362-1-AP(CHK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10362-1-AP(CHK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 10362-1-AP(CHK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10362-1-AP(CHK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP( CHK1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP( CHK1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of HepG2 cells using 10362-1-AP(Chk1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

1X10^6 HepG2 cells were stained with 0.2ug CHK1 antibody (10362-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.