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Chk1 Antibody 15 Publications

Rabbit Polyclonal| Catalog number: 10362-1-AP

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Con: 36 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Chk1 Antibody WB mouse thymus tissue 10362-1-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10362-1-AP(CHK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Chk1 Antibody IHC human lung cancer tissue 10362-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP( CHK1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Chk1 Antibody IHC human lung cancer tissue 10362-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10362-1-AP( CHK1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Chk1 Antibody IF HepG2 cells 10362-1-AP

Immunofluorescent analysis of HepG2 cells using 10362-1-AP(Chk1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Chk1 Antibody FC HepG2 cells 10362-1-AP

1X10^6 HepG2 cells were stained with 0.2ug CHK1 antibody (10362-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse thymus tissue, HeLa cells, K-562 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Chk1 fusion protein Ag0409
Full name:
CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight:
54 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC004202
Gene ID (NCBI):
1111
Gene symbol
CHEK1
Synonyms
CHEK1, Chk1

Background

In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated(PMID:22941630) and ubiquitinated (PMID:19276361). It has 3 isoforms produced by alternative splicing with the molecular weight of 54 kDa, 44 kDa and 50 kDa.


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