Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, mouse lung tissue, HeLa cells, A431 cells, mouse kidney tissue
|Positive IP detected in
|Positive IHC detected in
|mouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15761-1-AP targets CHMP1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|CHMP1A fusion protein Ag8389
|chromatin modifying protein 1A
|Calculated molecular weight
|196 aa, 22 kDa
|Observed molecular weight
|33 kDa, 25 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).
|Product Specific Protocols
|WB protocol for CHMP1A antibody 15761-1-AP
|IHC protocol for CHMP1A antibody 15761-1-AP
|IF protocol for CHMP1A antibody 15761-1-AP
|IP protocol for CHMP1A antibody 15761-1-AP
|Click here to view our Standard Protocols
Nat Cell Biol
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
J Extracell Vesicles
Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation
A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.
The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.
Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.