• Featured Product
  • KD/KO Validated

CHMP1A Polyclonal antibody

CHMP1A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15761-1-AP

Synonyms

CHMP1, CHMP1A, chromatin modifying protein 1A, hVps46 1, KIAA0047, PCOLN3, PRSM1, Vps46 1



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, mouse kidney tissue, mouse lung tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15761-1-AP targets CHMP1A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP1A fusion protein Ag8389
Full Name chromatin modifying protein 1A
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight 33 kDa, 25 kDa
GenBank accession numberBC007527
Gene symbol CHMP1A
Gene ID (NCBI) 5119
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).

Protocols

Product Specific Protocols
WB protocol for CHMP1A antibody 15761-1-APDownload protocol
IHC protocol for CHMP1A antibody 15761-1-APDownload protocol
IF protocol for CHMP1A antibody 15761-1-APDownload protocol
IP protocol for CHMP1A antibody 15761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Methods Mol Biol

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Authors - Shai Adar-Levor
mouseWB

Biochem Biophys Res Commun

miR-17 regulates the proliferation and differentiation of retinal progenitor cells by targeting CHMP1A.

Authors - Na Sun
human,mouseIHC

Cell Rep

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.

Authors - Michael E Coulter
  • KO Validated
humanWB

Cell Rep

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Authors - Inna Goliand
  • KD Validated
mouseWB

Nat Commun

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

Authors - Luana L Scheffer
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier