|Positive WB detected in||HEK-293 cells, A431 cells, HeLa cells, mouse kidney tissue, mouse lung tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||mouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15761-1-AP targets CHMP1A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHMP1A fusion protein Ag8389|
|Full Name||chromatin modifying protein 1A|
|Calculated molecular weight||196 aa, 22 kDa|
|Observed molecular weight||33 kDa, 25 kDa|
|GenBank accession number||BC007527|
|Gene ID (NCBI)||5119|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).
|Product Specific Protocols|
|WB protocol for CHMP1A antibody 15761-1-AP||Download protocol|
|IHC protocol for CHMP1A antibody 15761-1-AP||Download protocol|
|IF protocol for CHMP1A antibody 15761-1-AP||Download protocol|
|IP protocol for CHMP1A antibody 15761-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Methods Mol Biol
Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.
Biochem Biophys Res Commun
miR-17 regulates the proliferation and differentiation of retinal progenitor cells by targeting CHMP1A.
The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.
Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.
Nat Cell Biol
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.