Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat heart tissue, HeLa cells|
|Positive IHC detected in||human oesophagus cancer tissue, human heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13683-1-AP targets CHMP4B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHMP4B fusion protein Ag4544|
|Full Name||chromatin modifying protein 4B|
|Calculated molecular weight||224 aa, 25 kDa|
|Observed molecular weight||29-33 kDa|
|GenBank accession number||BC033859|
|Gene ID (NCBI)||128866|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C).
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes
Nat Cell Biol
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.
Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.
Ca2+-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sharad (Verified Customer) (08-30-2019)
The antibody works well in IF staining 1:500 dilution.