CHMP4B Polyclonal antibody

• Featured Product
• KD/KO Validated

CHMP4B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

58

Reviews (1)

Cat no : 13683-1-AP

Synonyms

SHAX1, hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B

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Validation Data Gallery

• 

  WB analysis of mouse heart using 13683-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13683-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13683-1-AP (CHMP4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13683-1-AP (CHMP4B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, HeLa cells
• Positive IHC detected in: human oesophagus cancer tissue, human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells, C2C12 cells, H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 21 publications below
• IF: See 40 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Product Information

13683-1-AP targets CHMP4B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHMP4B fusion protein Ag4544
• Full Name: chromatin modifying protein 4B
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-33 kDa
• GenBank Accession Number: BC033859
• Gene Symbol: CHMP4B
• Gene ID (NCBI): 128866
• RRID: AB_2877971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Publications

Species	Application	Title
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	IF	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
		Neuron; Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.; Authors - Juliet Goldsmith
human	CoIP	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	IF	Sci Adv; Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.; Authors - Virginia Andrade

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sharad (Verified Customer) (08-30-2019)

The antibody works well in IF staining 1:500 dilution.

• Applications: Immunofluorescence,
• Cell Tissue Type: RPE-1

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