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  • KD/KO Validated

CHMP4B Polyclonal antibody

CHMP4B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13683-1-AP

Synonyms

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue, HeLa cells
Positive IHC detected inhuman oesophagus cancer tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13683-1-AP targets CHMP4B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP4B fusion protein Ag4544
Full Name chromatin modifying protein 4B
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 29-33 kDa
GenBank accession numberBC033859
Gene symbol CHMP4B
Gene ID (NCBI) 128866
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C).

Protocols

Product Specific Protocols
WB protocol for CHMP4B antibody 13683-1-APDownload protocol
IHC protocol for CHMP4B antibody 13683-1-APDownload protocol
IF protocol for CHMP4B antibody 13683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanIF

Cell

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

Authors - Suresh Kumar
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier

Neuron

Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.

Authors - Juliet Goldsmith
humanIF

Sci Adv

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Authors - Virginia Andrade
humanIF

Nat Commun

Ca2+-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair.

Authors - Patrick Niekamp

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sharad (Verified Customer) (08-30-2019)

The antibody works well in IF staining 1:500 dilution.

  • Applications: Immunofluorescence,
  • Cell Tissue Type: RPE-1