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  • KD/KO Validated

CHMP4B Polyclonal antibody

CHMP4B Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 13683-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

C20orf178, chromatin modifying protein 4B, Chromatin-modifying protein 4b, hSnf7-2, hVps32-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inC2C12 cells, mouse heart tissue, rat heart tissue, HeLa cells, mouse brain tissue
Positive IF/ICC detected inC2C12 cells, H9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13683-1-AP targets CHMP4B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4544

Product name: Recombinant human CHMP4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC033859

Sequence: MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM

Predict reactive species
Full Name chromatin modifying protein 4B
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight25-33 kDa
GenBank Accession NumberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
RRIDAB_2877971
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Protocols

Product Specific Protocols
WB protocol for CHMP4B antibody 13683-1-APDownload protocol
IF protocol for CHMP4B antibody 13683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanIF

Cell

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

Authors - Suresh Kumar
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier

Neuron

Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.

Authors - Juliet Goldsmith
humanCoIP

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
humanIF

Sci Adv

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Authors - Virginia Andrade

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Sharad (Verified Customer) (08-30-2019)

The antibody works well in IF staining 1:500 dilution.

  • Applications: Immunofluorescence,
  • Cell Tissue Type: RPE-1
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