CHMP4B Polyclonal antibody

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CHMP4B Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 13683-1-AP Copied!

Publications (90)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

C20orf178, chromatin modifying protein 4B, Chromatin-modifying protein 4b, hSnf7-2, hVps32-2

• 

  WB analysis using 13683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 13683-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 13683-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 13683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of C2C12 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of H9C2 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of C2C12 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C2C12 cells, mouse heart tissue, rat heart tissue, HeLa cells, mouse brain tissue
• Positive IF/ICC detected in: C2C12 cells, H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13683-1-AP targets CHMP4B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4544

  Product name: Recombinant human CHMP4B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-224 aa of BC033859

  Sequence: MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM

• Full Name: chromatin modifying protein 4B
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-33 kDa
• GenBank Accession Number: BC033859
• Gene Symbol: CHMP4B
• Gene ID (NCBI): 128866
• RRID: AB_2877971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H444
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Publications

What published studies show

The CHMP4B Polyclonal antibody (Cat# 13683-1-AP) has accumulated 90 citations across high-impact journals including Nature, Science, Cell, Autophagy, Nature Communications, Cell Reports, EMBO Journal, and PLoS Biology. Associated research fields span ESCRT-III-mediated membrane repair, cytokinetic abscission, lysosomal repair, autophagy, endosomal trafficking, exosome biogenesis, cancer biology, neurodegeneration, viral infections, ferroptosis, and cardiovascular disease.

Automatically generated from the 90 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	IF	Nature; Stress granules plug and stabilize damaged endolysosomal membranes; Authors - Claudio Bussi
human	IF	Science; A p62-dependent rheostat dictates micronuclei catastrophe and chromosome rearrangements; Authors - Sara Martin
human	IF	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar
mouse		Immunity; Progranulin deficiency-induced lysosomal dysfunction drives maladaptive myeloid cell states through the MITF/TFE transcription factors; Authors - Leon Tejwani
human	WB	Nat Cell Biol; The Rubicon-WIPI axis regulates exosome biogenesis during ageing; Authors - Kyosuke Yanagawa

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single available review rates this antibody 4 out of 5 stars. The reviewer reported successful use in immunofluorescence staining on RPE-1 cells at a 1:500 dilution, noting that the antibody performed well under these conditions. Overall, the feedback is positive, confirming reliable performance for IF applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Sharad (Verified Customer) (08-30-2019)

The antibody works well in IF staining 1:500 dilution.

• Applications: Immunofluorescence,
• Cell Tissue Type: RPE-1