Claudin 4-specific Antibody

Claudin 4-specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16195-1-AP

Synonyms

claudin 4, CLDN4, CPE R, CPE receptor, CPER, CPETR, CPETR1, hCPE R, WBSCR8



Tested Applications

Positive WB detected inmouse small intestine tissue, human placenta tissue, MDA-MB-453s cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16195-1-AP targets Claudin 4-specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name claudin 4
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_001305
Gene symbol CLDN4
Gene ID (NCBI) 1364
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 4.

Protocols

Product Specific Protocols
WB protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IHC protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IP protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Gene Regul Mech

The chromatin remodeling protein BRM regulates the transcription of tight junction proteins: Implication in breast cancer metastasis.

Authors - Yuyu Yang
mouseIHC

Int J Mol Med

PMID: 32626909

Authors - Jianghong Gao
mouseWB,IHC

Am J Physiol Renal Physiol

Endotoxemia alters tight junction gene and protein expression in the kidney.

Authors - Eadon Michael T MT
humanWB

Cancer Sci

Targeting claudin-4 enhances chemosensitivity in breast cancer.

Authors - Yi Luo