|Positive WB detected in||mouse small intestine tissue, human placenta tissue, MDA-MB-453s cells|
|Positive IP detected in||human placenta tissue|
|Positive IHC detected in||human breast cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16195-1-AP targets Claudin 4-specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||claudin 4|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||NM_001305|
|Gene ID (NCBI)||1364|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 4.
|Product Specific Protocols|
|WB protocol for Claudin 4-specific antibody 16195-1-AP||Download protocol|
|IHC protocol for Claudin 4-specific antibody 16195-1-AP||Download protocol|
|IP protocol for Claudin 4-specific antibody 16195-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Endotoxemia alters tight junction gene and protein expression in the kidney.
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