Claudin 4-specific Polyclonal antibody

• 

  WB analysis of MDA-MB-453s using 16195-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 16195-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16195-1-AP (CLDN4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16195-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16195-1-AP (CLDN4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 16195-1-AP

  IP result of anti-Claudin 4-specific (IP:16195-1-AP, 4ug; Detection:16195-1-AP 1:300) with human placenta tissue lysate 4400ug.

• 

  IHC staining of human breast cancer using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 16195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Claudin 4-specific antibody (16195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 16195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Claudin 4-specific antibody (16195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Claudin 4-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 16195-1-AP

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Synonyms

Claudin 4, CLDN4, CPE R, CPE receptor, CPER

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, mouse small intestine tissue, human placenta tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 33 publications below
• IHC: See 15 publications below
• IF: See 11 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

16195-1-AP targets Claudin 4-specific in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: claudin 4
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_001305
• Gene Symbol: Claudin 4
• Gene ID (NCBI): 1364
• RRID: AB_2082969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against Claudin 4. Claudin 4 is overexpressed in a variety of cancers. It can be used as a predictor of recurrent malignant pleural effusion in patients with advanced non-small cell lung cancer (PMID: 38629624).

Publications

Species	Application	Title
mouse,human	IHC,IF	Cell Metab; Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes; Authors - Junpeng Yang
rat	WB	Gut Microbes; S-amlodipine induces liver inflammation and dysfunction through the alteration of intestinal microbiome in a rat model; Authors - Xinxin Liu
rat,mouse	WB,IHC	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
human	WB	Free Radic Biol Med; Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.; Authors - Restituto Tocmo
human	WB,RIP	Cancers (Basel); Targeting PVT1 Exon 9 Re-Expresses Claudin 4 Protein and Inhibits Migration by Claudin-Low Triple Negative Breast Cancer Cells.; Authors - Fayola Levine
rat	WB	EBioMedicine; Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis.; Authors - Yingchun Luo