Mast Cell Chymase Polyclonal antibody

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  WB analysis of mouse heart using 18189-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18189-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18189-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18189-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18189-1-AP

  IP result of anti-Mast Cell Chymase (IP:18189-1-AP, 4ug; Detection:18189-1-AP 1:300) with mouse heart tissue lysate 2400ug.

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  IHC staining of human tonsillitis using 18189-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 18189-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Mast Cell Chymase Polyclonal Antibody for WB, IP, ELISA

Cat No. 18189-1-AP

Publications(14)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Synonyms

Alpha chymase, chymase, chymase 1, mast cell, CMA1, CYH, CYM, Mast Cell Chymase, Mast cell protease I, Mcpt 5, Mcpt5, MCT1

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human placenta tissue, MCF-7 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 4 publications below

Product Information

18189-1-AP targets Mast Cell Chymase in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Mast Cell Chymase fusion protein Ag12909
• Full Name: chymase 1, mast cell
• Calculated Molecular Weight: 247 aa, 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC069370
• Gene Symbol: Mast Cell Chymase
• Gene ID (NCBI): 1215
• RRID: AB_2083611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23946
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mast Cell Chymase, also named as CYH, CYM and CMA1, belongs to the peptidase S1 family and Granzyme subfamily. It is the major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. CMA1 maybe a target for cardiovascular disease therapies.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.; Authors - Emily Mackey
human	WB	Arterioscler Thromb Vasc Biol; Stabilization of Perivascular Mast Cells by Endothelial CNP (C-Type Natriuretic Peptide).; Authors - Wen Chen
human		Int J Mol Sci; Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.; Authors - Mika Yamanaka-Takaichi
human	WB	Cell Cycle; Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.; Authors - Haoqing Shi
mouse	WB	Sci Rep; Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.; Authors - Nicole Meyer
mouse	WB	Immunology; The circadian clock is functional in eosinophils and mast cells.; Authors - Baumann Anja A

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (02-14-2025)

MCC (in green) does not seem to have worked specifically on the epileptic human FFPE cortex. It is supposed to mark only mast cells, but it seems to have some puncta-like staining of the neurons (in red) too.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Mast Cell Chymase Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:18189-1-AP)