WB result from Dr. Vishram Kedar. Lysates from transfected HEK293 cells as indicated in the figure above, were subjected to the western bloting using rabbit CNOT1 antibody (Cat # 14276-1-AP) from Protein-Tech group, Inc, at dilution 1:1000 in 5% non-fat dry milk/TBST, followed by goat anti-rabbit HRP (Bio-RAD) at the dilution of 1:10,000 in 5% non-fat dry milk/TBST. Only bands of the size of 174 kDa were seen. 267 kDa bands of CNOT1 were not visible in this short exposure.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human brain tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human heart slide using 14276-1-AP( CNOT1 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded human heart tissue slide using 14276-1-AP( CNOT1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical of paraffin-embedded human kidney using 14276-1-AP(CNOT1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human kidney using 14276-1-AP(CNOT1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human spleen using 14276-1-AP(CNOT1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human spleen using 14276-1-AP(CNOT1 antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-CNOT1 (IP:14276-1-AP, 5ug; Detection:14276-1-AP 1:500) with mouse kidney tissue lysate 4000ug.