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  • KD/KO Validated

CNOT1 Polyclonal antibody

CNOT1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 14276-1-AP


AD 005, CCR4 associated factor 1, CDC39, CNOT1, hNOT1, KIAA1007, NOT1, NOT1H

Tested Applications

Positive WB detected inhuman brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells
Positive IP detected inmouse kidney tissue, HeLa cells
Positive IHC detected inhuman heart tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14276-1-AP targets CNOT1 in WB, IP, IF, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623
Full Name CCR4-NOT transcription complex, subunit 1
Calculated Molecular Weight 267 kDa
Observed Molecular Weight 240-250 kDa, 174 kDa
GenBank Accession NumberBC040523
Gene Symbol CNOT1
Gene ID (NCBI) 23019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.


Product Specific Protocols
WB protocol for CNOT1 antibody 14276-1-APDownload protocol
IHC protocol for CNOT1 antibody 14276-1-APDownload protocol
IF protocol for CNOT1 antibody 14276-1-APDownload protocol
IP protocol for CNOT1 antibody 14276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Authors - Masashi Yamaji


Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Authors - Leppek Kathrin K


The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Aditya (Verified Customer) (06-20-2024)

Exactly how I would expect an antibodty to behave. Excellent. There is a nonspecific band at 100KDa, but this is probably some variant of CNOT1, so cant fault this at all.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: hek293

Roy (Verified Customer) (06-12-2024)

It does detect CNOT1 by WB from HeLa extracts ( 1/1000 dilution - Overnight incubation at 4°C). It works very well to Immunoprecipitate CNOT1 from both cytoplasmic and nuclear fractions of HeLa cells (4µg /IP)

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1/1000 (WB)
  • Cell Tissue Type: HeLa

aditya (Verified Customer) (10-25-2022)

This is brilliant. We use this for cnot1 pull downs and then use a seperate cnot1 antibody to image on the western. Great for identifying interacting partners.

  • Applications: Western Blot, Protein purification
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Hek293

Tobias (Verified Customer) (10-25-2022)

  • Applications: Western Blot, Immunoprecipitation

Parisa (Verified Customer) (10-17-2022)

good quality, high sensitivity

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293

Will (Verified Customer) (01-31-2019)

good antibody, would use again

  • Applications: Western Blot,