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  • KD/KO Validated

CNOT1 Polyclonal antibody

CNOT1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 14276-1-AP

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, hNOT1, KIAA1007, NOT1, NOT1H



Tested Applications

Positive WB detected inhuman brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14276-1-AP targets CNOT1 in WB, IP, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623
Full Name CCR4-NOT transcription complex, subunit 1
Calculated molecular weight 267 kDa
Observed molecular weight 240-250 kDa, 174 kDa
GenBank accession numberBC040523
Gene symbol CNOT1
Gene ID (NCBI) 23019
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.

Protocols

Product Specific Protocols
WB protocol for CNOT1 antibody 14276-1-APDownload protocol
IHC protocol for CNOT1 antibody 14276-1-APDownload protocol
IP protocol for CNOT1 antibody 14276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA

Recruitment of the 4EHP-GYF2 cap-binding complex to tetraproline motifs of tristetraprolin promotes repression and degradation of mRNAs with AU-rich elements.

Authors - Rui Fu
mouseWB

Life Sci Alliance

CSDE1 controls gene expression through the miRNA-mediated decay machinery.

Authors - Pavan Kumar Kakumani
humanWB

Cell Rep

Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis.

Authors - Toshimichi Yamada
humanWB

PLoS One

The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex.

Authors - Doidge Rachel R
  • KD Validated
humanWB

Mol Biol Cell

The cAMP pathway regulates mRNA decay through phosphorylation of the RNA-Binding Protein TIS11b/BRF1.

Authors - Felicitas Rataj
humanWB

Cell Signal

CNOT7/hCAF1 is involved in ICAM-1 and IL-8 regulation by tristetraprolin.

Authors - Jia-Xin Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Will (Verified Customer) (01-31-2019)

good antibody, would use again

  • Applications: Western Blot,